A fully automated method has been developed for the determination of total cholesterol in blood serum, using the AutoAnalyzer system. According to the new method, based on the cholesterol determination of Abell et a!. (1), the serum sample is saponified and subsequently extracted with carbon tetrachloride. After treatment with Liebermann-Burchard reagent, the amount of cholesterol is determined...

The main purpose of this study in order to estimate fat soluble vitamins contents Mackerel fillets from the northeastern coast of Tunisia. Retinol, α-tocophérol and cholecalciferol were analyzed using HPLC system. The experimental procedure used for extraction consisted on saponification, normal phase separation with UV and fluorescence detection for tocopherol and retinol. While cholecalcifero...

"adipocere" we only find one publication which speaks of this change being observed in a warm country, i.e., " Eight cases of Saponification " by S. C. Mackenzie (.Indian Medical Gazette, 1889, XXIV, p. 42). The author was Police Surgeon at Calcutta, and claimed that during 9 years lie observed 8 cases of adipocere-formation in bodies of persons drowned in the Hooghlj^, or buried in damp soil. ...

Wastes produced during fermentation and distillation of crude sugarcane juice in rum factories were evaluated as a new source of waxes. The chemical composition of the crude wax extracted from adsorbat of the wastes on fuller's earth was studied by GC-mass spectrometry. Series of linear alkanes (C19-C33), and wax esters constitute the main components. In addition, phytosterols, triterpene methy...

Acid mucopolysaccharides in mast cell granules were histochemically studied in the lesion of urticaria pigmentosa and in the dermis of normal human skin. Alcian blue and azure A were used to stain mucopolysaccharides. Bromphenol blue was employed for detection of basic proteins. In a further attempt to identify various polyanions, staining was carried out with alcian blue containing various con...

In this study, we combined different physico-chemical methods to show the effect of overheating on the quality of golden fry cooking oil. Those methods include spectroscopic methods such us 1H-NMR, 13C-NMR and FTIR. Results showed that both overheating and excessive reuse of golden fry cooking oil seriously affected its quality by increasing its density, viscosity and different indexes like ref...

A simple stereoselective synthesis of montroumarin [(3S)-6,8-dihydroxy-3-phenyl-3,4-dihydroisocoumarin] isolated from Montrouziera sphaeroidea has been achieved. Condensation of benzoyl chloride with 3,5-dimethoxyhomophthalic acid afforded 6,8-dimethoxy-3-phenylisocoumarin (3) which on sequential saponification and esterification yielded the keto ester 5. Enantioselective reduction of the latte...

A new product implicated in cereal grain polysaccharide cross-linking has been authenticated by independent synthesis. Saponification of cereal grain fiber releases the RRRS/SSSR-isomer of 2,5-bis-(4-hydroxy-3-methoxyphenyl)-tetrahydrofuran-3,4-dicarboxylic acid. The parent ester logically derives from 8-8-coupling of ferulate followed by water addition to one of the incipient quinone methide m...