We describe a fluorogenic two-site noncompetitive heterogeneous immunoassay with magnetic beads on a low-voltage digital microfluidic platform using closed electrowetting-on-dielectric (EWOD). All the steps of an enzyme-linked immunosorbent assay (ELISA) were performed on the device using 9H-(1, 3-dichloro-9, 9-dimethylacridin-2-one-7-yl) phosphate as the fluorogenic substrate for the enzyme al...

Soil salinization is detrimental to crop growth, agricultural yields, and environmental protection. Echinochloa frumentacea (Roxb.) Link a pioneer species for the alteration of saline–alkali lands. In this paper, we examined effects intercropping between E. leguminous forages on saline land improvement in soil Hetao-Ningxia Plain, China. We found that increased diversity richness arbuscular myc...

sYNoPsis Bilateral adrenalectomy produces a reduction in the alkaline phosphatase concentration in rat intestine, which is not prevented by the administration of saline, but is prevented by the administration ofcortisone. The administration of A.C.T.H. to normal rats leads to a rise in intestinal alkaline phosphatase levels associated with a small increase in the weight of the adrenals, while t...

Denture hygiene is essential because denture biofilm is involved in oral infections and systemic diseases. Although there are chemical agents available on the market, none of them have ideal properties and research on such products is still necessary. The aim of this study was to evaluate the efficacy of a castor bean (Ricinus communis)-based solution for removing denture biofilm, compared to t...

In hippocampus, synchronous activation of CA1 pyramidal neurons causes a rapid, extracellular, population alkaline transient (PAT). It has been suggested that the plasma membrane Ca(2+)-ATPase (PMCA) is the source of this alkalinization, because it exchanges cytosolic Ca(2+) for external H(+). Evidence supporting this hypothesis, however, has thus far been inconclusive. We addressed this long-s...

[1] Jarosite occurs within altered tephra from the saline‐alkaline paleolake deposits of Pliocene‐Pleistocene Olduvai Gorge, Tanzania. Zeolites (mainly phillipsite), authigenic K‐feldspar, and Mg/Fe‐smectites dominate the mineral assemblage, indicating saline‐ alkaline diagenetic conditions (pH > 9). As jarosite is ordinarily an indicator of acidic conditions on Earth and Mars, its association ...

We have isolated a set of complementary DNA (cDNA) clones that together encode the alkaline phosphatase of human colon cancer LS174T cells. These clones include two cDNAs isolated from a conventionally prepared oligodeoxythymidylate-primed lambda ZAP cDNA library and three cDNA clones prepared by using the polymerase chain reaction. The deduced amino acid sequence of the alkaline phosphatase pr...