To the Editor: The last step in electrophoretic procedures for creatine kinase (CK; EC 2.7.3.2) isoenzyme separation on cellulose acetate is to incubate the plate with a reagent that will develop either fluorescence or color for each isoenzyme. In our laboratory we routinely use fluorescence to detect the isoenzymes. The reagent used must be highly sensitive, so that low proportions of the isoe...

background and objectives: the seeds of securigera securidaca (l.) deg. & dorf. (fabaceae) are used as anti-diabetic remedy in iranian folk medicine. the aim of the present study was to establish the callus and suspension culture of s. securidaca seeds for the first time and to determine the major secondary metabolites including cardiac glycosides and total phenols. methods: the culture of s. s...