Studies on the disposition of extracellular S-adenosylhomocysteine by isolated rat hepatocytes have shown that S-adenosyl-L-homocysteine is not taken up by cells, but binds to acceptor(s) on the cell surface. The Scatchard plots for the binding of S-adenosylhomocysteine to hepatocytes and purified rat liver membranes at 0 degrees were nonlinear, and consistent with high-affinity components with...

Various phosphosilicate doped polypyrrole proton conductive thin films composite membranes were successfully prepared. Phosphosilicate obtained by sol-gel process mixing tetraethoxy silane with phosphoric acid at a molar ratio 1:5. The conductivity increased increasing both the content of gel and relative humidity. Temperature dependence showed Vogel-Tamman-Fulcher type behavior, indicating tha...

To study behavior of activated G(alpha)(s) in living cells, green fluorescent protein (GFP) was inserted within the internal amino acid sequence of G(alpha)(s) to generate a G(alpha)(s)-GFP fusion protein. The fusion protein maintained a bright green fluorescence and was identified by immunoblotting with antibodies against G(alpha)(s) or GFP. The cellular distribution of G(alpha)(s)-GFP was sim...

addition of n-butyl nitrite to isolated rat hepatocytes caused an immediate glutathione depletion followed by an inhibition of mitochondrial respiration, inhi- bition of glycolysis and atp depletion. at cytotoxic butyl nitrite concentrations, lipid  peroxidation  occurred  before  the  plasma  membrane  was  disrupted. cytochrome p-450 inhibitors inhibited peroxynitrite formation and prevented ...

S,S,S-tris(2-ethylhexyl) phosphorotrithioate proved to be an effective solvent mediator for constructing a mexiletine-sensitive membrane electrode in combination with an ion-exchanger, sodium tetrakis[3,5-bis(2-methoxyhexafluoro-2-propyl)phenyl]borate. Among a series of phosphorus compounds containing phosphoryl (P=O) groups, this solvent mediator showed the highest sensitivity to mexiletine in...

OBJECTIVES The mode of action of dysgalacticin, a large (21.5 kDa), heat-labile bacteriocin that is active against the human pathogen Streptococcus pyogenes, was investigated. METHODS We used recombinant dysgalacticin to determine its mode of action against S. pyogenes. Antimicrobial activity of dysgalacticin was determined by MIC assays and viability counts. The extracellular pH of glucose-e...

isolation and purification of major outer membrane proteins (omp) from the cell wall envelope of brucella abortus s-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (tx-100) and lysozyme treatments, followed by ultracentrifugation. the crude omp was treated with trypsin to free the preparation from any other protein contaminants. t...

New bionanocomposite proton exchange membranes were prepared from sulfonated polybenzimidazole (s-PBI) and various amounts of sulfonated ZnO/cellulose nanohybrids (ZnO/cellulose-SO3H). The use of ZnO/cellulose-SO3H compensates for the decrease in ion exchange capacity of membranes observed when non-sulfonated nano-fillers are utilized. The strong –SO3H/–SO<su...

the aim of our work was to compare cytotoxic and membrane-damaging effects of o. majorana l. essential oil and linalool on hep g2 and to investigate their possible protective (antioxidant) effects against h2o2 induced membrane damage. the oils were investigated by gc and gc-ms and antioxidant activity was evaluated by dpph and -carotene–linoleic acid assays. assessment of cell viability was ma...

background: the purpose of this study was to investigate the efficiency of flat sheet membrane bioreactor (fs-mbr) in bod 5 and cod removal of isfahan south municipal wastewater treatment plant. methods: in this study a submerged fs-mbr, with 140 liters volume was fed using microfiltration membrane (mf) during 109 days and hydraulic retention time of 20 hours. findings: the mbr application in m...