Introduction Tumor progression and growth require an appropriate rate of blood vessel formation for the rate of neoplastic cellular proliferation. Angiogenesis is regulated by the balance of proand anti-angiogenic factors. The most potent pro-angiogenic factor is vascular endothelial growth factor (VEGF). Its expression is regulated by pathways related to the normal physiologic response to hypo...

Deletion of TIEG1/KLF10 in mice results in a gender specific osteopenic skeletal phenotype with significant defects in both cortical and trabecular bone, which are observed only in female animals. Calvarial osteoblasts isolated from TIEG1 knockout (KO) mice display reduced expression levels of multiple bone related genes, including Runx2, and exhibit significant delays in their mineralization r...

The Runx2 (Cbfa1/AML3) transcription factor and matrix metalloproteinase 9 (MMP9) are key regulators of growth plate maturation and bone formation. The genes for both proteins are characteristic markers of breast and prostate cancer cells that metastasize to bone. Here we experimentally addressed the compelling question of whether Runx2 and MMP are functionally linked. By cDNA expression array ...

AIM To investigate clinical significance of runt-related transcription factor (RUNX)-2 in epithelial ovarian cancer (EOC). METHODS RUNX2 protein expression and its subcellular localization were detected by immunohistochemistry in 116 patients with EOC. RESULTS RUNX2 protein was predominantly expressed in cell nucleus of EOC tissues. The expression level of RUNX2 in EOC tissues was significa...

Prostate cancer patients with regional lymph node involvement at radical prostatectomy often experience disease progression to other organs, with the bone as the predominant site. The transcription factor Runx2 plays an important role in bone formation and prostate cancer cell migration, invasion, and metastasis. Here we showed that the forkhead box O (FOXO1) protein, a key downstream effector ...

The synthesis of type I collagen, the main component of bone matrix, precedes the expression of Runx2, the earliest determinant of osteoblast differentiation. We hypothesized that the energetic needs of osteoblasts might explain this apparent paradox. We show here that glucose, the main nutrient of osteoblasts, is transported in these cells through Glut1, whose expression precedes that of Runx2...

Introduction Genetic factors contribute to the aetiology of the ectopic canine (EC) (OMIM 189490), the inheritance pattern being autosomal dominant with variable expression and incomplete penetrance. However there is also evidence for an epigenetic component [1]. The Runx2 gene is intimately involved in the mechanism of tooth eruption. It controls differentiation and maturation of osteoblasts a...

There are many gene products reported to promote osteoblast differentiation and thus increase bone formation, but only the transcription factor Runx2 and members of the bone morphogenetic protein (BMP) family of growth/differentiation factors have been shown to be absolute requirements for osteogenesis. Mice lacking the transcription factor Runx2 (also known as cbfa1) develop no bone. Similarly...

Runx2 and phosphatidylinositol 3-kinase (PI3K)-Akt signaling play important roles in osteoblast and chondrocyte differentiation. We investigated the relationship between Runx2 and PI3K-Akt signaling. Forced expression of Runx2 enhanced osteoblastic differentiation of C3H10T1/2 and MC3T3-E1 cells and enhanced chondrogenic differentiation of ATDC5 cells, whereas these effects were blocked by trea...