The nature of the early RNA response was analyzed by polyacrylamide gel electrophoresis. The extraction procedure allowed quantitative isolation of undegraded RNA from whole mouse skin. By 3 hr after treatment with effective tumor promoters, there was a large stimulation of incorporation of cytidine-3H into "32 S" RNA, rRNA, and 4 to 5 S RNA. Stimulation of cytidine-3 H incorporation into 32 S ...

Existing methods rely primarily on heat to remove crosslinks and adducts, which is only partially effective and leads to additional fragmentation of labile nucleic acids such as RNA. In contrast, the catalytic CAT5TM technology developed by Cell Data Sciences and included in the RNAstormTM kit greatly accelerates the removal of formaldehyde damage and allows the use of milder conditions, result...