Cleavage of phosphodiester bonds by small ribonuclease mimics within different bulge-loops of RNA was investigated. Bulge-loops of different size (1-7 nt) and sequence composition were formed in a 3' terminal fragment of influenza virus M2 RNA (96 nt) by hybridization of complementary oligodeoxynucleotides. Small bulges (up to 4 nt) were readily formed upon oligonucleotide hybridization, wherea...

1. Ribonuclease II of Escherichia coli degrades pulse-labelled RNA associated with ribosomes and polyuridylic acid on ribosomes and in solution to mononucleotides. 2. Ribosomal and pulse-labelled RNA in solution and ribosomal RNA in chloramphenicol particles (protein-deficient ribosomes) are degraded to oligonucleotides. 3. Ribosomal RNA in mature ribosomes is not attacked by the enzyme. 4. Fro...

Ribonuclease B is a constituent of the zymogen granules of bovine pancreatic acinar cells and is secreted in the pancreatic juice of cattle (2). Its isolation from bovine pancreatic juice has been described (3). The enzyme is a glycoprotein that possesses an amino acid composition indistinguishable from that of ribonuclease A and contains carbohydrate to the extent of 6 residues of mannose and ...

The telegram that I received from the Swedish Royal Academy of Sciences specifically cites ". . . studies on ribonuclease, in particular the relationship between the amino acid sequence and the biologically active conformation. . . . The work that my colleagues and I have carried out on the nature of the process that controls the folding of polypeptide chains into the unique three-dimensional s...

A two-dimensional “mapping” procedure (1) with paper electrophoresis and paper chromatography permits the separation and identification of the mono-, di-, tri-, and tetranucleotides obtained by digestion of tobacco mosaic virus ribonucleic acid with pancreatic ribonuclease (2). This procedure has also been used for the analysis of micrococcal nuclease digests of deoxyribonucleic acid (3) by the...

The aim of this review is to draw a correlation between the structure of the DNArRNA hybrid and its properties as a substrate for the RNase H, as well as to point the crucial structural requirements for the modified AONs to preserve their RNase H potency. The review is divided Ž . Ž . into the following parts: 1 mechanistic considerations, 2 target RNA folding–AON folding– Ž . Ž . RNase H assis...

Replicative intermediate ribonucleic acid (RNA), designated RI, which contained parental RNA labeled with (32)P was separated by filtration through agarose from the nucleic acids prepared from (32)P-labeled RNA phage-infected Escherichia coli. A larger amount of ribonuclease-sensitive parental label was found in the rapidly sedimenting forms of RI than in the slower sedimenting forms, indicatin...

Ribonucleases represent a new class of antitumor RNA-damaging drugs. However, many wild-type members of the vertebrate secreted ribonuclease family are not cytotoxic because they are not able to evade the cytosolic ribonuclease inhibitor. We previously engineered the human pancreatic ribonuclease to direct it to the cell nucleus where the inhibitor is not present. The best characterized variant...

The telegram that I received from the Swedish Royal Academy of Sciences specifically cites ". . . studies on ribonuclease, in particular the relationship between the amino acid sequence and the biologically active conformation...” The work that my colleagues and I have carried out on the nature of the process that controls the folding of polypeptide chains into the unique three-dimensional stru...

Alterations of carbohydrate structures in cancer cells are the most promising targets for developing clinical diagnostic reagents. Pancreatic cancer is one of the most difficult cancers to diagnose because it lacks definitive symptoms. Two antibodies were raised against human pancreatic ribonuclease 1 that bind to the enzyme containing unglycosylated Asn(88), but not when its Asn(88) is N-glyco...