The present work evaluates the techno-economic feasibility of a rhamnolipids production process that utilizes digestate from anaerobic digestion (AD) food waste. Technical feasibility, profitability and extent investment risks between fermenter scale its operating strategy for was investigated in study. Three scenarios were generated compared: using single large (Scenario I), two small fermente...

Biodiesel side stream waste glycerol was identified as a cheap carbon source for rhamnolipids (RLs) production which at the same time could improve management of waste. The present study aimed to produce RLs by using Pseudomonas aeruginosa RS6 utilizing substrate and evaluate their physico-chemicals properties. Fermentation conditions such temperature, initial medium pH, concentration, nitrogen...

Vinasse, a residue from bioethanol production containing high organic matter concentration, was used as substrate in submerged fermentation of Pseudomonas aeruginosa PA1 for biosurfactant production. About 2.7 g/L of rhamnolipids was obtained, with surface tension of 29.2 mN/m and critical micelle concentration of 80.3 mg/L. After separation of rhamnolipid and biomass, residual fermentation med...

Biosurfactant activity and naphthalene degradation by a new strain identified as Bacillus cereus 28BN were studied. The strain grew well and produced effective biosurfactants in the presence of n-alkanes, naphthalene, crude oil and vegetable oils. The biosurfactants were detected by the surface tension lowering of the medium, thin layer chromatography and infrared spectra analysis. With (2%) na...

A low-cost microBR (microbioreactor) made from PTFE [poly(tetrafluoroethylene)] was used to cultivate a model organism, Pseudomonas aeruginosa DS10-129. The progress of bioprocessing was monitored by comparing the growth of the organism in a microBR, a conventional bench scale bioreactor and a shake flask. Under the microBR conditions, the organism produced 23 mg/ml of pyocyanin that had antimi...

The opportunistic pathogen Pseudomonas aeruginosa PAO1 has a remarkable capacity to adapt to various environments and to survive with limited nutrients. Here, we report the discovery and characterization of a novel small non-coding RNA: NrsZ (nitrogen-regulated sRNA). We show that under nitrogen limitation, NrsZ is induced by the NtrB/C two component system, an important regulator of nitrogen a...