نتایج جستجو برای: restriction enzyme
تعداد نتایج: 308199 فیلتر نتایج به سال:
Background and Objective: The glycoprotein hormone secreted from anterior pituitary gland are heterodimeric, non- covalently consisting of a common α subunit and a hormone-specific β subunit. Due to the lack of adequate access to hormones and the possibility of contamination, this study attempted to clone two subunits of LH hormone by using IRES sequence in eukaryotic expression vector pEGFP-NI...
Background and purpose: Toxoplasmosis is a common parasitic disease throughout the world and one-third of the population has antibodies to Toxoplasma gondii. This disease causes serious medical problems in fetuses and immunocompromised individuals. As gene encoding protein GRA14 can be considered as a suitable target for DNA vaccine and designing diagnostic kits the aim of this study was to ...
This study was conducted to detect the presence ofseJA gene among Salmonella Enteritidis isolates from poultry sources by polymerase chain reaction (Pf'R) and evaluate its potential as diagnostic and epidemiological tools. Thirty Salmonella isolates from poultry sources: broilers. broiler breeders, layers, hatcheries, and poultry abattoirs were investigated. Upper and forward primers were const...
use in restriction-enzyme analysis. This procedure is cost-effective because it saves the use of expensive miniprep kits for use with only positively identified recombinant clones and limits typically time-consuming miniprep steps. Our method can be accomplished in a few minutes in two microcentrifuge tubes with minimal enzyme, expense, no phenol/chloroform extractions and no ethanol precipitat...
Restriction enzyme profiles of group G beta-hemolytic streptococci associated with a point source outbreak and an outbreak of sporadic pharyngitis in two different communities were compared. To asses the epidemiologic utility of this approach for studying group G streptococci, DNA fingerprints of strains responsible for a point source outbreak of pharyngitis associated with the consumption of c...
Reports on the prevalence of abortion due to Chlamydia psittaci (Chlamydophila abortus) in Kerala have indicated a need for the in depth study of the organism. To unearth differences among the isolates of Chlamydia psittaci, at molecular level, restriction enzyme analysis of genomic DNA and plasmid profiling were carried out. Four isolates from four different sources were used in this study. DN...
The restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5 ́AT↓CGAT 3 ́, was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37oC and over a wide range of pH and salt...
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