nitric oxide (no) is an important signalling molecule with diverse physiological functions inplants. in the last years, many advances have been obtained regarding no synthesis and its physiologicaleffects in plants. it was found to play a crucial role in plant growth and development, starting fromgermination to flowering, ripening of fruit and senescence of organs, respiratory metabolism, as we...

The control of the fructose 2,6-bisphosphate (Fru2,6P(2)) concentration and its possible role in controlling carbohydrate synthesis and degradation are discussed. This regulator metabolite is involved in the fine tuning of photosynthetic metabolism, and in controlling photosynthetic partitioning, and may also be involved in the response to hormones, wounding, and changing water relations. Study...

Tiglyglycine (TG), an intermediate product of the catabolism of isoleucine, is increased in the urine of patients with beta-ketothiolase deficiency or with disorders of propionate metabolism. It is also implicated as a useful diagnostic marker in disorders of the respiratory chain. We present a method for the synthesis of TG and tiglyl[13C, 15N]glycine and the development of a stable isotope di...

Human neutrophils prepared from fresh blood were activated with either latex o r latex plus phorbol myristate acetate. Phagolysosomes subsequently isolated by gradient centrifugation were enriched 6or 4-fold, respectively, in respiratory burst activity as measured by superoxide dismutase-sensitive NADPH-cytochrome c reductase activity. The phagolysosomes were also enriched about 4-fold in ubi...

Cystic fibrosis is a mono-genetic multi-system disease; however, respiratory manifestations cause the main morbidity and mortality where chronic bacterial infections lead to bronchiectasis and ultimately respiratory failure. Metabolomics allows a relatively complete snapshot of metabolic processes in a sample using different mass spectrometry methods. Sample types used for discovery of biomarke...

Physiologic and environmental factors can modulate antibiotic activity and thus pose a significant challenge to antibiotic treatment. The quinolone class of antibiotics, which targets bacterial topoisomerases, fails to kill bacteria that have grown to high density; however, the mechanistic basis for this persistence is unclear. Here, we show that exhaustion of the metabolic inputs that couple c...