Many strains of E. coli K12 restrict DNA containing cytosine methylation such as that present in plant and animal genomes. Such restriction can severely inhibit the efficiency of cloning genomic DNAs. We have quantitatively evaluated a total of 39 E. coli strains for their tolerance to cytosine methylation in phage and plasmid cloning systems. Quantitative estimations of relative tolerance to m...

Loss of bronchodilator effectiveness or tolerance has been observed with inhaled beta-agonists but not with inhaled anticholinergic medications. Initially, tolerance is reflected in loss of bronchial protection against stimuli followed by loss of bronchodilator properties. However, generally such observations have been reported in asthma. A 6-month randomized, double-dummy placebo-controlled tr...

seeds of nine sugar beet lines: five drought tolerant, two susceptible, and two medium tolerant were washed, sterilised, dried, and sown in water agar medium for germination test. seedling growth characters such as cotyledon fresh weight (cfw), cotyledon dry weight (cdw), root fresh weight (rfw), root dry weight (rdw), and root length (rl) were determined after the end of 28 days period of expe...

To investigate the role of dehydrins (DHNs) in extreme low-temperature (LT) tolerance, we sampled needle tissue of Siberian spruce (Picea obovata Ledeb.) from trees growing in an arboretum in Trondheim, Norway from August 2006 to April 2007 and tracked changes in LT tolerance via relative electrolyte leakage. We used western blotting to estimate relative amounts of proteins binding a DHN K-segm...

We consider several basic classes of tolerance relations among objects. These (global) relations are deened from some predeened similarity measures on values of attributes. A tolerance relation in a given class of tolerance relations is optimal with respect to a given decision table A if it contains only pairs of objects with the same decision and the number of such pairs contained in the relat...

To investigate the impact of Xuefu Zhuyu decoction on cardiac remodeling and quality life patients with heart failure middle-range coronary disease ejection fraction is main objective study. 116 in middle range were divided as observation or control group. Clinical efficacy both groups was observed changes traditional Chinese medicine symptom scores, function classification, exercise tolerance,...

In this study we present a simple optimization model for the evolution of defensive strategies (tolerance and resistance) of plants against their natural enemies. The model specifically evaluates the consequences of introducing variable costs and benefits of tolerance and resistance and nonlinear cost-and-benefit functions for tolerance and resistance. Incorporating these assumptions, the prese...

Improving drought tolerance through drought preconditioning is an important way to understand drought tolerance mechanism in plants. Some limited researches have focused on the gene expression patterns in conjunction with the underlying enzymes promoting drought tolerance and post-drought recovery in white clover. The objective of this study was to identify whether preconditioning-induced droug...