Ismail Kadare, the most famous writer of today's Albanian literature, is also one writers contemporary literature in general. Therefore, whenever we mention this genius belles-letters, first all our mind goes to his work, its structure, themes, characters, actions and events, words messages, course, artistic features. Although it rare come across works any other author a literary approach that ...

Dr. Seyed Taghi Noorbakhsh died in a tragic car accident on Nov 15th 2018, while on an official mission in Golestan Province in Iran. There is a great emptiness left by Dr.Noorbakhsh’s passing. He will be missed by his colleagues andthousands of his patients whom he helped during his career.In addition to being a board member of Iranian Orthopedic Association (IOA), for consecutive terms & Chie...

Nicholas J. Strausfeld was born in Surrey and raised in Brighton, Sussex. He received his B. Sc. and Ph.D. from University College London (1965, 1968) and his German Habilitation from the University of Frankfurt. After 19 years in Germany, first at Frankfurt’s Zoology Institute, later the MPI Tübingen and EMBL Heidelberg, he was appointed full professor in 1987 in the Division of Neurobiology a...

[59] Milman Parry’s work attempted to demonstrate the traditional character of Homeric poetry. Despite a later shift in focus from the concept of “traditional” to “oral” (Finnegan 1991: 120), with at least the implication of a relatively open system as opposed to a relatively closed one, Parry’s early emphasis on tradition was so strong that his theory tended to be read as one discounting, if n...

We celebrate the centenary of the Nobel award to Elie Metchnikoff in 2008, shared with Paul Ehrlich, as respective pioneers of cellular and humoral immunology. Metchnikoff is rightly famous for his recognition of the biological significance of leukocyte recruitment and phagocytosis of microbes in host defence against infection, inflammation and immunity. As a comparative zoologist he utilised a...

BACKGROUND A versatile strategy for protein-surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriacetic acid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free, non-invasive monitoring of molecule adsorption/desorption at surfaces. RESULTS This paper describes a SE stu...

Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged bovine rhodopsin. These quantities were obtained with the recombinant baculovirus/Sf9 insect cell-based expression system in large-scale bioreactor cultures with the use of a serum-free and protein-f...

The crystal structure of a mutant form of the single-chain fragment (scFv), derived from the monoclonal anti-His tag antibody 3D5, in complex with a hexahistidine peptide has been determined at 2.7 A resolution. The peptide binds to a deep pocket formed at the interface of the variable domains of the light and the heavy chain, mainly through hydrophobic interaction to aromatic residues and hydr...

The cDNA of the human GM2-activator protein was cloned into the expression vector pHX17. The plasmid encodes a fusion protein with a hexahistidine tail and a Factor Xa cleavage site at its N-terminus. The recombinant protein was purified from cell homogenates under denaturing conditions by metal-ion affinity chromatography in a single step and then was refolded. The hexahistidine tail could be ...