risk of HDFN, which can cause fetal anemia, hydrops, and intrauterine fetal death. Babies born with HDFN are at significant risk of neonatal morbidity and mortality [1,2]. Prenatal detection of the fetal RhD status in a pregnant woman with RhD-negative provides useful information for predicting the risk of HDFN and Noninvasive fetal RHD genotyping using cell-free fetal DNA incorporating fetal R...

This focused review presents a critical appraisal of the World Heart Federation criteria for the echocardiographic diagnosis of rheumatic heart disease (RHD) and its performance in African RHD screening programmes. It identifies various logistical and methodological problems that negatively influence the current guideline's performance. The authors explore novel RHD screening methodology that c...

Background and Aims: Influenza is an acute respiratory illness that is caused by a virus belonging to Orthomyxoviridae family. This virus spreads rapidly every year in cold season and leads to morbidities and mortalities especially in adults and children, which causes billions of dollars of economic losses. Accordingly, development of a rapid, sensitive and inexpensive laboratory diagnosis base...

Abstract BACKGROUND: Diagnosis of Echinococcus granulosus in the definitive host particularly in dog is the significant complication in the endemic area. OBJECTIVES: The aim of this study is serological detection of E. granulosus in the infected dogs. METHODS: Dot-ELISA based on the copro-antigen and recombinant EPC1 antigen (rEPC1) for antibody detection was performed. Blood and fecal samples ...

OBJECTIVES To assess the accuracy of fetal RHD genotyping using cell-free fetal DNA in maternal plasma at different gestational ages. DESIGN A prospective multicentre cohort study. SETTING Seven maternity units in England. PARTICIPANTS RhD negative pregnant women who booked for antenatal care before 24 weeks' gestation. INTERVENTIONS Women who gave consent for fetal RHD genotyping had b...

BACKGROUND The knowledge of D variants in patients and donors is important because anti-D alloimmunization can occur in some but not all individuals who express a variant RHD allele. Serologic distinction of RhD discrepancies is not always straightforward, which makes molecular analysis highly desirable. METHODS A group of 223 subjects, 129 patients, and 94 blood donors was identified and ana...

BACKGROUND AND PURPOSE Rheumatic heart disease (RHD) is an important risk factor for ischemic stroke in developing countries. Observational data on its characteristics and influence on outcome or recurrence of ischemic stroke are scarce. We aimed to investigate proportions, characteristics, functional outcome, and recurrence of acute ischemic stroke patients with RHD in a hospitalized Chinese p...

OBJECTIVE  To determine the accuracy of non-invasive fetal testing for the RHD gene in week 27 of pregnancy as part of an antenatal screening programme to restrict anti-D immunoglobulin use to women carrying a child positive for RHD DESIGN:  Prospectively monitoring of fetal RHD testing accuracy compared with serological cord blood typing on introduction of the test. Fetal RHD testing was perfo...

background and objectives: the obligate intracellular bacterium chlamydia trachomatis causes sexually transmissible diseases in human. timely and sensitive detection of this pathogen is very important. there are many cross-reactions in bacteriological and serological methods in detection of this type of pathogens. the aim of this study was to achieve a more specific antigen for serological test...

BACKGROUND RhD discrepancies between current and historical results are problematic to resolve. The investigation of 10 discrepancies is reported here. STUDY DESIGN Samples identified were those that reacted by automated gel technology and were negative with an FDA-approved reagent. Reactivity with a commercially available panel of monoclonal anti-D was performed. Genomic DNA was evaluated fo...