Recombinant Mycobacterium bovis BCG expressing foreign antigens represents a promising candidate for the development of future vaccines and was shown in several experimental models to induce protective immunity against bacterial or parasitic infections. Innate resistance to BCG infection is under genetic control and could modify the immune responses induced against an antigen delivered by such ...

BACKGROUND Antibodies to soluble liver antigen/liver pancreas (SLA/LP) are specific markers of autoimmune hepatitis. Their target antigen has recently been cloned. AIMS To establish standardised immunoassays using the recombinant antigen, and to assess the frequency and significance of seropositivity in patients from different countries. METHODS An enzyme linked immunoassay was developed us...

results primary, secondary, and 3d structure of cchfv gn were obtained and pcr reaction with m13 forward and reverse primers confirmed the generation of recombinant bacmid dna harboring gn coding region under polyhedron promoter. conclusions characterization of the detailed structure of cchfv gn by bioinformatics software provides the basis for development of new experiments and construction of...

PURPOSE Currently, therapeutic tumor vaccines under development generally lack significant effects in human clinical trials. Exploring a powerful antigen delivery system is a potential approach to improve vaccine efficacy. We sought to explore engineered bacterial outer membrane vesicles (OMVs) as a new vaccine carrier for efficiently delivering tumor antigens and provoking robust antitumor imm...

Advances in diagnostic assays for parasitic diseases include the use of monoclonal antibodies (MAbs) in antigen capture and competitive inhibition enzyme-linked immunosorbent assays (C-ELISA). Antigen capture ELISAs for Anaplasma marginale and Cryptosporidium parvum provide direct detection of these parasites during clinical disease, and the C-ELISA format has been adapted for detection of anti...

OBJECTIVE This study was to clone, identify and analyze the characteristics of egG1Y162 gene from Echinococcus granulosus. METHODS Genomic DNA and total RNAs were extracted from four different developmental stages of protoscolex, germinal layer, adult and egg of Echinococcus granulosus, respectively. Fluorescent quantitative PCR was used for analyzing the expression of egG1Y162 gene. Prokaryo...

The nucleocapsid (N) protein of the Toscana (TOS) virus was expressed in Escherichia coli by using a pET15b vector. The recombinant protein was purified by affinity chromatography and was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and enzyme immunoassay (EIA). The recombinant antigen was reactive with positive human sera, and the reactivity corre...

P H A S E 1 and phase-2 antigen types of a diphasic salmonella strain are determined by distinct genes, H I and H,, respectively (LEDERBERG and EDWARDS 1953; LEDERBERG and IINO 1956). They are nonallelic and are transduced independently. Therefore, an H-antigen type recombinant obtained by a transduction usually expresses the antigen type of the donor in one phase, but retains that of the recip...

Serology remains the method of choice for laboratory diagnosis of Mycoplasma pneumoniae infection. Currently available serological tests employ complex cellular fractions of M. pneumoniae as antigen. To improve the specificity of M. pneumoniae diagnosis, a recombinant protein was assessed as a serodiagnostic reagent. A panel of recombinant proteins were expressed from a cloned M. pneumoniae gen...

I? HASE-1 and phase-2 antigen types of a diphasic salmonella strain are determined by distinct genes, H, and H,, respectively (LEDERBERG and EDWARDS 1953; LEDERBERG and IINO 1956). They are nonallelic and are transduced independently. Therefore, an H-antigen type recombinant obtained by a transduction usually expresses the antigen type of the donor in one phase, but retains that of the recipien...