Dpm1 is the structural gene for mannosylphospho dolichol synthase (i.e., Dol-P-Man synthase, DPMS) in S. cerevisiae. Earlier studies with cDNA cloning and sequence analysis have established that Mr 31 kDa DPMS of S. cerevisiae contains a consensus sequence (YRRVIS) that can be phosphorylated by cAMP-dependent protein kinase (PKA). We have been studying the upregulation of DPMS activity by PKA-m...

background: streptokinase is a bacterial protein produced by different beta hemolytic streptococci and widely used in thrombolytic treatment. the main disadvantage of using streptokinase is antibody formation which causes allergic reaction to neutralize effects of streptokinase therapy. aim of this study was investigate of recombinant mutant streptokinase fibrinolytic activity. m aterials and m...

Background: Alkaline pectate lyase plays an important role in papermaking, biological refining and wastewater treatment, but its industrial applications are largely limited owing to low activity poor alkali resistance. Methods: The alkaline BspPel from Bacillus RN.1 was heterologously expressed Escherichia coli BL21 (DE3) resistance were improved by loop replacement. Simultaneously, the effect ...

Proteases are closely associated with many pathological conditions. Efficient detection of protease activity may be useful for diagnosis, prognosis, and the development new therapeutic biomolecules. Fluorescent Resonance Energy Transfer (FRET) is defined as non-radioactive energy transfer that occurs between two fluorophores. proteins widely used in FRET biosensors because they can genetically ...

Evidence for the role of chitinases in biocontrol by Trichoderma species has been well documented.Chit42 lacks a chitin–binding domain (ChBD) which is involved in its binding activity to insoluble chitin. The objective of the present study was to enhance antifungal activity of T. harzianum by overexpression of wild type and hybrid forms of Chit42 containing chitin binding domain. To produce chi...

Mammalian phenylalanine hydroxylase (PAH) catalyses the conversion of L-phenylalanine to L-tyrosine in the presence of dioxygen and tetrahydrobiopterin; it is a highly regulated enzyme. Little is known about the rates of synthesis and degradation of PAH in vivo. The enzyme has been reported to have a half-life of approx. 2 days in rat liver and 7-8 h in rat hepatoma cells, but the mechanism of ...

The complete glcK gene from the fish pathogen Renibacterium salmoninarum, encoding a glucose kinase, was analyzed and expressed. The partial characterization of the recombinant enzyme confirmed that it belongs to a group of glucose kinases involved in carbon catabolite repression. Multiple sequence alignments were used to deduce a new consensus sequence for this family of bacterial proteins, ch...

enzyme preparations purified from either recombinant strain of E. coli or B. subtilis were shown to be identical with each other as regards the profile of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and were composed of a single peptide with the molecular size of 70 kDa. Determination of the amino-terminal sequence of the two enzyme preparations revealed that both amino-terminal s...

Nitrate analysis in water is one of the most frequently applied methods in environmental chemistry. Current methods for nitrate are generally based on toxic substances. Here, we show that a viable alternative method is to use the enzyme nitrate reductase. The key to applying this Green Chemistry solution for nitrate analysis is plentiful, inexpensive, analytical grade enzyme. We demonstrate tha...