objective: antigen 85 complex of mycobacterium tuberculosis includes three immunogenic proteins which are important tb vaccine candidates. the use of these protein as a component of subunit vaccines, as a part of dna vaccines or recombinant bcg boosters, enhances their recombinant production. recombinant production of these mycobacterial proteins located in the cell wall somehow differs from th...

  Objective(s):  Infection by high-risk papillomavirus is regarded as the major risk factor in the development of cervical cancer. Recombinant DNA technology allows expression of the L1 major capsid protein of HPV in different expression systems, which has intrinsic capacity to self-assemble into viral-like particles (VLP). VLPS are non-infectious, highly immunogenic and can elicit neutralizing...

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background helicobacter pylori multiplies and causes infection in human gastric mucosal layer. new approaches have focused on using specific treatments, such as immunotherapy, to limit this infection. urease, as one of the most important virulent and antigenic factors of the bacterium, is a suitable target for this purpose. patients and methods in order to prepare recombinant proteins, the synt...

taq dna polymerase is widely used in laboratories and for this reason many investigators have focused their attention on understanding the role of various regions and amino acids in this enzyme. o-helix is a part of taq polymerase suggested to play an important role in the enzyme fidelity. the influence of asn666 in this helix on the enzyme function has never been investigated, and therefore by...

helicobacter pylori infection is among the most prevalent infections in the world involving more than half the adult population. h. pylori infection results in active chronic gastritis, peptic ulcers and enhances the risk of gastric malignancies. it is of utmost importance to prevent h. pylori infection particularly in highly prevalent countries including iran. the urease holoenzyme produced by...