BACKGROUND & OBJECTIVES immunocompromized adults. Approximately 50 per cent of the GBS strains carry and express the gene of BAC antigen which is capable to bind IgA. Gene encoding for the BAC antigen has been cloned and sequenced but actual IgA binding region on the protein has not been detected. The aim of the present work was to localize the region of IgA binding on Bac protein, to evaluate ...

Neurocysticercosis (NCC) is an important cause of neurological disease worldwide. A 10-kDa antigen of Taenia solium metacestodes (TsMs) has been shown to be specific for immunodiagnosis of NCC. Screening of a TsM complementary DNA (cDNA) library isolated a cDNA encoding this protein. The cloned cDNA contained a 258-bp complete open-reading frame that encodes an 86-amino acid polypeptide with a ...

A truncated recombinant 56-kDa outer membrane protein of the Karp strain of Orientia tsutsugamushi (Kp r56) was evaluated in cynomolgus monkeys (Macaca fascicularis) for immunogenicity and safety as a vaccine candidate for the prevention of scrub typhus. This recombinant antigen induced strong humoral and cellular immune responses in two monkeys and was found to be well tolerated. Antigen-speci...

Two recombinant antigens (P22U and PLA2), cloned in a L4 Dirofilaria immitis cDNA library, were analyzed by Western-blot and ELISA to investigate their characteristics for the diagnosis of human dirofilariosis. P22U seems related to a Di22 native antigen useful for the diagnosis of pulmonary dirofilariosis, but it is unspecifically recognized by sera from patients with different parasitic and n...

In order to investigate the crucial role of ZmBT1 in starch accumulation during maize grain development and analyze expression distribution various tissues, we prepared a polyclonal antibody. Specifically, successfully expressed recombinant plasmid pGEX-6p-ZmBT1-C (382-437aa) purified Gst-ZmBT1-C as antigen for antibody preparation. Our results confirmed that protein tissues can be specifically...

background: during the last two decades, significant advances have been made in the fields of lactococcal genetics and protein expression. lactococcus lactis (l. lactis) is an effective vector for protein expression and can be used as an antigen delivery system. hence, l. lactis is an ideal candidate for mucosal immunotherapy. profilin (che a 2), the major allergen in chenopodium album, is one ...