نتایج جستجو برای: recombinant allergen
تعداد نتایج: 122341 فیلتر نتایج به سال:
BENCHMARKS Immunoglobulin E (IgE)-mediated allergic diseases have become a serious health problem in industrialized countries (1,2). Routine diagnosis of allergic conditions is based on clinical history, skin test reactivity, and serum IgE determinations against the offending agents (3). Both skin test reactivity and determinations of serum IgE levels reflect directly on the quality of the al-l...
PURPOSE The scientific experiments with new immunological methods (immunoblotting, RAST inhibition) and isolation of recombinant allergens suggest structural similarities in the allergenic components responsible for cross-reactions. Immunochemical and molecular biology studies indicate that epitopes of major allergen (Bet v 1, Mal d 1) contain more IgE binding epitopes than minor allergens (Bet...
A CZE-ESI-TOF MS method has been optimized for the separation and identification of nitrated variants of the major birch pollen allergen from Betula verrucosa, isoform 1a (Bet v 1a). In-house nitration of recombinant Bet v 1a was done by peroxynitrite. As a BGE, 10 mmol/L ammonium bicarbonate with pH 7.50 provided best resolution. Nebulizer gas pressure and sheath liquid flow rate of 0.4 bar an...
BACKGROUND The house dust mite (HDM) allergen Der p 18 belongs to the glycoside hydrolase family 18 chitinases. The relevance of Der p 18 for house dust mite allergic patients has only been partly investigated. OBJECTIVE To perform a detailed characterization of Der p 18 on a molecular, structural and immunological level. METHODS Der p 18 was expressed in E. coli, purified to homogeneity, t...
Aim. Selection and combination of immunodiagnostic tests to detect active tuberculosis (TB) in children with concomitant bronchopulmonary pathologies. Materials methods. A prospective study adolescents ( n = 236) was conducted, which included cases localized pulmonary tuberculosis; associated allergic or infectious inflammatory forms chronic nonspecific lung diseases (CNSLD); under a significan...
from either grass or tree pollens, insect venoms, cat dander as well as house dust mites [2–13] ( fig. 1 ). Such fusion proteins can then be expressed in Escherichia coli , yeasts (e.g. Saccharomyces cerevisiae or Pichia pastoris ) and plant or mammalian host cell systems prior to purification and refolding. One advantage, obviously, is to produce the multiallergen drug substance as a single mo...
Methods We evaluated subjects from Southern Romania, a Central European region with temperate continental climate with submediterranean and humid subtropical influences, where sensitization to Betulaceae pollen is less important in pollinosis compared with that to grass or weed pollen. We selected adult patients from the region of sylvosteppe with low density forests dominated by deciduous spec...
from either grass or tree pollens, insect venoms, cat dander as well as house dust mites [2–13] ( fig. 1 ). Such fusion proteins can then be expressed in Escherichia coli , yeasts (e.g. Saccharomyces cerevisiae or Pichia pastoris ) and plant or mammalian host cell systems prior to purification and refolding. One advantage, obviously, is to produce the multiallergen drug substance as a single mo...
PURPOSE To characterize the roles played by monocyte chemoattractant protein-1 and its preferential receptor CCR2 (MCP-1/CCL2) in acute allergic inflammation. METHODS The direct effects of MCP-1 were evaluated histologically after a subconjunctival injection of recombinant MCP-1 into naïve mice. The mice were sensitized to ragweed pollen, and allergic conjunctivitis was induced by an allergen...
Allergen-specific immunotherapy is the only allergen-specific and disease-modifying treatment for allergy. The construction and characterization of a vaccine for birch pollen allergy is reported. Two nonallergenic peptides, PA and PB, derived from the IgE-reactive areas of the major birch pollen allergen Bet v 1 were fused to the hepatitis B surface protein, PreS, in four recombinant fusion pro...
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