نتایج جستجو برای: recombinant allergen

تعداد نتایج: 122341  

Journal: :BioTechniques 2003
Claudio Rhyner Zoltan Konthur Kurt Blaser Reto Crameri

BENCHMARKS Immunoglobulin E (IgE)-mediated allergic diseases have become a serious health problem in industrialized countries (1,2). Routine diagnosis of allergic conditions is based on clinical history, skin test reactivity, and serum IgE determinations against the offending agents (3). Both skin test reactivity and determinations of serum IgE levels reflect directly on the quality of the al-l...

Journal: :Roczniki Akademii Medycznej w Bialymstoku 2005
B Cudowska M Kaczmarski P Restani

PURPOSE The scientific experiments with new immunological methods (immunoblotting, RAST inhibition) and isolation of recombinant allergens suggest structural similarities in the allergenic components responsible for cross-reactions. Immunochemical and molecular biology studies indicate that epitopes of major allergen (Bet v 1, Mal d 1) contain more IgE binding epitopes than minor allergens (Bet...

2013
Sergey Gusenkov Chloé Ackaert Hanno Stutz

A CZE-ESI-TOF MS method has been optimized for the separation and identification of nitrated variants of the major birch pollen allergen from Betula verrucosa, isoform 1a (Bet v 1a). In-house nitration of recombinant Bet v 1a was done by peroxynitrite. As a BGE, 10 mmol/L ammonium bicarbonate with pH 7.50 provided best resolution. Nebulizer gas pressure and sheath liquid flow rate of 0.4 bar an...

2016
Yvonne Resch Katharina Blatt Ursula Malkus Christian Fercher Ines Swoboda Margit Focke-Tejkl Kuan-Wei Chen Susanne Seiberler Irene Mittermann Christian Lupinek Azahara Rodriguez-Dominguez Petra Zieglmayer René Zieglmayer Walter Keller Vladislav Krzyzanek Peter Valent Rudolf Valenta Susanne Vrtala

BACKGROUND The house dust mite (HDM) allergen Der p 18 belongs to the glycoside hydrolase family 18 chitinases. The relevance of Der p 18 for house dust mite allergic patients has only been partly investigated. OBJECTIVE To perform a detailed characterization of Der p 18 on a molecular, structural and immunological level. METHODS Der p 18 was expressed in E. coli, purified to homogeneity, t...

Journal: : 2023

Aim. Selection and combination of immunodiagnostic tests to detect active tuberculosis (TB) in children with concomitant bronchopulmonary pathologies. Materials methods. A prospective study adolescents ( n = 236) was conducted, which included cases localized pulmonary tuberculosis; associated allergic or infectious inflammatory forms chronic nonspecific lung diseases (CNSLD); under a significan...

2015

from either grass or tree pollens, insect venoms, cat dander as well as house dust mites [2–13] ( fig. 1 ). Such fusion proteins can then be expressed in Escherichia coli , yeasts (e.g. Saccharomyces cerevisiae or Pichia pastoris ) and plant or mammalian host cell systems prior to purification and refolding. One advantage, obviously, is to produce the multiallergen drug substance as a single mo...

2014
Mariana Vieru Florin-Dan Popescu Adriana Tudose Nicolae Dumitrescu

Methods We evaluated subjects from Southern Romania, a Central European region with temperate continental climate with submediterranean and humid subtropical influences, where sensitization to Betulaceae pollen is less important in pollinosis compared with that to grass or weed pollen. We selected adult patients from the region of sylvosteppe with low density forests dominated by deciduous spec...

2015

from either grass or tree pollens, insect venoms, cat dander as well as house dust mites [2–13] ( fig. 1 ). Such fusion proteins can then be expressed in Escherichia coli , yeasts (e.g. Saccharomyces cerevisiae or Pichia pastoris ) and plant or mammalian host cell systems prior to purification and refolding. One advantage, obviously, is to produce the multiallergen drug substance as a single mo...

Journal: :Investigative ophthalmology & visual science 2009
Takeshi Tominaga Dai Miyazaki Shin-ichi Sasaki Sachiko Mihara Naoki Komatsu Keiko Yakura Yoshitsugu Inoue

PURPOSE To characterize the roles played by monocyte chemoattractant protein-1 and its preferential receptor CCR2 (MCP-1/CCL2) in acute allergic inflammation. METHODS The direct effects of MCP-1 were evaluated histologically after a subconjunctival injection of recombinant MCP-1 into naïve mice. The mice were sensitized to ragweed pollen, and allergic conjunctivitis was induced by an allergen...

Journal: :Journal of immunology 2013
Katharina Marth Isabella Breyer Margarete Focke-Tejkl Katharina Blatt Mohamed H Shamji Janice Layhadi Anna Gieras Ines Swoboda Domen Zafred Walter Keller Peter Valent Stephen R Durham Rudolf Valenta

Allergen-specific immunotherapy is the only allergen-specific and disease-modifying treatment for allergy. The construction and characterization of a vaccine for birch pollen allergy is reported. Two nonallergenic peptides, PA and PB, derived from the IgE-reactive areas of the major birch pollen allergen Bet v 1 were fused to the hepatitis B surface protein, PreS, in four recombinant fusion pro...

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