The recombinant antibody H90-10 detects the endogenous human heat-shock protein 90 beta (Hsp90β) by immunoprecipitation (IP) and Western blotting.

The recombinant antibodies AS739, AT693 and AU734 detect by immunofluorescence the spike S protein from SARS-CoV-2.

Synthesis of Trimethyl Chitosan Nanoparticles Containing Recombinant BLF1-stxB Protein Burkholderia Pseudomallei and Evaluation its Immunogenicity in Mice

objective(s):multi-epitopic protein vaccines and direction of vaccine delivery to dendritic cells (dcs) are promising approaches for enhancing immune responses against mutable pathogens. escherichia coli is current host for expression of recombinant proteins, and it is important to optimize expression condition. the aim of this study was the optimization of multi-epitopic hiv-1 tat/pol/gag/env ...

human alpha 1-antitrypsin (aat) cdna was obtained from hepg2 cell lines. after pcr and construction of expression vector ppiczα-aat, human aat was expressed in the yeast pichia pastoris (p.pastoris) in a secretary manner and under the control of inducible alcohol oxidase 1 (aox1) promoter. the amount of aat protein in medium was measured as 60 mg/l 72 hr after induction with methanol. results i...

background: cryptosporidium parvum is a parasitic protozoan that functions as important causa­tive agent of diarrhea in human and animals. the host's immune response to surface anti­gens of c. parvum has been previously demonstrated. in this respect, the role of humoral immu­nity in the development of host protective immunity against this protozoon has been well demon­strated. methods: the effe...

heat stress is a major environmental stress limiting wheat productivity in most cereal growing areas of the world. in order to map and characterize quantitative trait loci controlling heat tolerance, 144 recombinant inbred lines deriving from the cross of kauz and mtrwa116 were assessed in a greenhouse and growth chamber at 35°c. one hundred and sixty six ssr and 3 aflp markers were used to...

background: several studies reported the clinical features of ige-mediated hypersensitivity after ingestion of melon. melon allergy is a common ige-mediated fruit allergy in iran. this prompted us to investigate immunochemical and molecular properties of the major allergen in melon fruit, to compare the ige-binding capacity of the natural protein with the recombinant allergen, and to determine ...

the genome of hb v virus of serotype ayw cloned in pbr322 and expression shuttle vector p yes2 were used for construction of the hbsag chimeric genes and their expression in saccharomyces cerevisiae. two recombinant plasmids were constructed. one of them contained the coding sequences for the major polypeptide of surface antigen. another construct carried the major polypeptide with the pre-s2 a...