The expression of an antigen on porcine T lymphocytes detected by murine monoclonal antibody (mAbl 8/1 was investigated by functional studies and dual-parameter immunofluorescence. mAb 8/1 reacts with >95% of thymocytes and in peripheral blood with all T lymphocytes and with cells of the monocyte/macrophage lineage, but not with B cells, erythrocytes, and platelets. Pretreatment of peripheral b...

INTRODUCTION To study the presence of European bat lyssavirus (EBLV) infections in bat reservoirs in Sweden, active surveillance was performed during the summers from 2008 to 2013. MATERIAL AND METHODS Bat specimens were collected at >20 bat colonies in the central, southeastern, and southern parts of Sweden. In total, blood and saliva of 452 bats were examined by a virus neutralization test ...

The effect of IdX-specific rabbit and allogeneic antiidiotype antibodies (Ab2) was investigated in vivo in Igh-Cb mouse strains with respect to the induction of a cross-reactive idiotype (IdX)-positive anti-alpha (1-3) Dextran (Dex) response. These C.B20 and C57Bl/6 mice have an allotype-linked incapacity to respond with IdX-positive anti-alpha (1-3) Dex antibodies upon conventional immunizatio...

Rotavirus (RV) and norovirus (NoV) are the two major causes of viral gastroenteritis (GE) in children worldwide. We have developed an injectable vaccine design to prevent infection or GE induced with these enteric viruses. The trivalent combination vaccine consists of NoV capsid (VP1) derived virus-like particles (VLPs) of GI-3 and GII-4 representing the two major NoV genogroups and tubular RV ...

Anti-idiotype antisera were raised in syngeneic (BALB/c mice) and homologous (A/J mice) systems to study the cross-reactive idiotypes among monoclonal antibodies to PR8 and B/Lee virus HA and the expression of these idiotypes during primary and secondary antiviral responses of BALB/c mice. Extensive idiotypic cross-reactivity was demonstrated among monoclonal antibodies specific for distinct an...

A panel of monoclonal antibodies reactive with normal lymphocyte subsets was used to classify cases of lymphocytic leukemia on the basis of cell surface antigen expression. The antibodies employed were commercially available and included a common framework HLA-DR antibody, two pan-T antibodies (Leu-1 and OKT-3), and antibodies defining cytotoxic/suppressor (Leu-2 and OKT-8) and helper/inducer (...

Antibody-dependent enhancement (ADE) is an important safety concern for vaccine development against dengue virus (DENV) and its antigenically related Zika (ZIKV) because may prime deleterious antibodies to enhance natural infections. Cross-reactive targeting the conserved fusion loop epitope (FLE) are known as main sources of ADE. We design ZIKV immunogens engineered change FLE conformation but...

Immunological characteristics of recombinant fragments of the Plasmodium falciparum blood-stage antigen Pf332 Halima A. Balogun Stockholm 2011 ii These pictures were taken during inhibition assay with rabbit antibodies; they are infected and non-infected red cells, stained with acridine orange. The picture above in collaboration with another infected red cell, formed the parasites probably beca...

The Present study attempts to determine the relationship between proliferation and inhibition of apoptosis in normal endometrium hyperplasia, using monoclonal antibodies against marker, Ki-67 anti-apoptotic protein, Bcl-2 expression B-cell lymphoma 2 (Bcl – 2) Ki 67 cyclical proliferative secretory phase. includes Bcl endometrial hyperplasia.