Large-scale codon re-encoding is a new method of attenuating RNA viruses. However, the use of infectious clones to generate attenuated viruses has inherent technical problems. We previously developed a bacterium-free reverse genetics protocol, designated ISA, and now combined it with large-scale random codon-re-encoding method to produce attenuated tick-borne encephalitis virus (TBEV), a pathog...

Voice signal Goals Cryptographic key for cryptographic applications Main criteria • Key (re)generation for user should be reliable and efficient on resource-constrained devices • Key search should be difficult for attacker, even with captured device

conclusions our study demonstrated that the b1 gene, more so than the re gene, showed positive samples and can be used to detect toxoplasmosis, although the b1 gene, in comparison to the re gene, did not show any superiority of molecular diagnosing capability. results also showed that toxoplasma molecular detection methods can be used instead of routine serological detection methods in a clinic...

thinking simultaneously of humor and battlefield is hardly expectable; however, the most genuine jokes can be seen in this very battlefield verbal culture in a simple and sensitive form which has rarely been paid attention to. this witticism can be categorized into four classes; namely, devotional, fight and martyrdom, drinking and eating, and social interactions which have the most similitude ...

Background: The aim of this study was evaluation of developmental rate and Bax, Bcl-2 and ErbB4 genes expression following re-vitrification in compact and early blastocysts stages. Materials and Methods: 5-8 cell embryos were collected from female mature mice, 60-62 hours post hCG injection. The embryos were divided to five groups including: fresh, vitrified at 5-8 cells, vitrified at blastocys...

This paper describes surge, a syntactic realization front-end for natural language generation systems. By gradually integrating complementary aspects of various linguistic theories within the computational framework of functional unii-cation, surge has evolved to be one of the most comprehensive grammars of English for language generation available today. It has been successfully re-used in a v...