A rapid neutralization test for influenza A and B viruses was developed. In this method, a 96-well tissue culture plate was used for the preparation of cell monolayers and the peroxidase-antiperoxidase staining technique was used for the visualization of foci infected with these viruses. In the presence of trypsin and tragacanth gum, clear foci developed 1 day after infection. A linear relation...

BACKGROUND Rabies is a major public health problem with a fatality rate close to 100%; however, complete prevention can be achieved through pre- or post-exposure prophylaxis. The rapid fluorescent focus inhibition test (RFFIT) is one of the recommended testing methods to determine the production of neutralizing antibodies after vaccination. Here, we report the development of a new monoclonal an...

background and objectives: pseudomonas aeruginosa is one of the most important opportunistic pathogens in patients who suffer from immunodeficiency and burns. wound infections caused by p. aeruginosa can rapidly change to systemic and life threatening situation. the aim of this study was to apply florescent in situ hybridization (fish) method for rapid detection of p. aeruginosa in burrn patien...

Inhibition of cytochrome P450 (CYP450) enzymes can result in potential clinical drug-drug interaction liabilities. Because of the safety and economic concern leading to clinical failure or product withdrawal, screening of lead molecules for CYP450 inhibition potential is nowadays conducted much earlier in the drug development process. At this early phase, a large number of lead molecules are in...

Introduction: Various factors are involved in the pathogenesis of acne vulgaris. Recently, G6PD deficiency has been proposed in the pathogenesis of acne. G6PD has an important role in the oxidant/antioxidant balance. According to this theory, antioxidants are used in the treatment of acne recently. The aim of this study was to evaluate G6PD deficiency in patient with acne vulgaris. Methods: The...

There are three approaches to aflatoxin detection in corn: (1) a presumptive test that can be done in the field to determine whether a corn lot should be analyzed for aflatoxin or not; (2) rapid screening methods to establish the presence or absence of toxin: and (3) quantitative methods to detennine toxin levels. The presumptive test for aflatoxin in corn is the black light test or the bright ...

Ochratoxin A (OTA) is a mycotoxin identified as a contaminant in grains and wine throughout the world, and convenient, rapid and sensitive detection methods for OTA have been a long-felt need for food safety monitoring. Herein, we presented a new competitive format based lateral flow strip fluorescent aptasensor for one-step determination of OTA in corn samples. Briefly, biotin-cDNA was immobil...

A rapid (6-7 hr), modified Kirby-Bauer disk-susceptibility method, by which derivatives of tetrazolium dyes are used to enhance delineation between areas of growth and zones of inhibition, has been developed. Inoculated petri plates, prepared by the Kirby-Bauer method, were sprayed, after 6 to 7 hr of incubation (37 C), with aqueous solutions of MTT-tetrazolium or INT-tetrazolium resulting in r...