Limonium dufourii (Plumbaginaceae) is a triploid species with obligate apomictic reproduction and is endemic to the East Mediterranean coast of Spain, where it is present in only six populations, most of which have a very low number of individuals. Genetic variation and population structure in this species was studied using amplified fragment length polymorphisms (AFLPs) as markers, using the s...

Like any other branch of Agricultural Sciences, the Division of Nematology at IARI, initiated researches in the exciting field of biochemistry and molecular biology of plant parasitic nematodes during the early seventies of last century. The main research areas were biochemical mechanism of resistance against phytonematodes and diagnostics of economically important plant parasitic nematodes. Sp...

The use of PCR based fingerprinting using short primers is very sensitive to variation in procedures. Strict laboratory procedures, exceeding general molecular biology standards, must be followed. Many laboratories have experienced difficulties in obtaining reproducible banding. Using a stock solution greatly minimizes pipetting small quantities, but the stability of a Taq stock is not well kno...

Leyland's cypress (xCupressocyparis leylandii) is a putative, spontaneous hybrid between Chamaecyparis nootkatensis and Cupressus macrocarpa. To investigate this putative origin, twenty five Leyland's cypresses were sampled, along with living, putative parents of Ch. nootkatensis and Cup. macrocarpa from gardens in the UK. The DNA was extracted and analyzed by DNA fingerprinting (RAPDs) and Int...

The spontaneous fasciation mutation generates novel developmental diversity in cultivated soybean, Glycine max (L.) Merrill. An increased apical dominance in the mutant inhibits axillary buds, causes a branchless phenotype, and restricts reproduction to shoot apices. The fasciation mutation is encoded by a recessive (f) allele at a single locus. The mutation, despite its importance in soybean d...

Relative afferent pupillary defects (RAPDs) in amblyopia have been reported, and it is widely accepted that amblyopes can have an RAPD. We investigated whether or not this could be confirmed by the use of binocular pupillography. We examined twelve patients (6 males and 6 females, aged 7-57 years) with unilateral amblyopia associated with anisometropia and/or strabismus, using binocular infrare...

Considerable concern has been voiced regarding the reproducibil i ty/transferabil i ty of RAPT) markers across different genetic backgrounds in genetic mapping experiments. Therefore, separate gametic subsets (mapping populations) were used to construct individual random amplified polymorphic DNA (RAPD) linkage maps for a single longleafpine (Pinuspalwtris Mill.). A haploid mapping population c...

The development of molecular techniques for genetic analysis has led to a great increase in our knowledge of cereal genetics and our understanding of the structure and behaviour of cereal genomes. These molecular techniques, in particular the use of molecular markers, have been used to monitor DNA sequence variation in and among the species and create new sources of genetic variation by introdu...

The invasive alien knotweeds, Fallopia spp. (Polygonaceae), are some of the most troublesome invasive species in Europe and North America. Invasive success in Fallopia may be enhanced by multiple hybridization events. We examined the pattern of hybridization and its evolutionary consequences in Belgium with a concerted analysis of ploidy levels (chromosome counts and flow cytometry), morphologi...

UNLABELLED PREMISE Studies of hybridizing species are facilitated by the availability of species-specific molecular markers for identifying early- and later-generation hybrids. Cattails are a dominant feature of wetland communities, and a better understanding of the prevalence of hybrids is needed to assess the ecological and evolutionary effects of hybridization. Hybridization between Typha...