Staphylococcus aureus (S. aureus) is a Gram-positive bacterium that causes wide range of variable illnesses and can be either transmitted from one place to another through contact with patients or airborne. Because the increased diversity S. globally, particularly those are clinical significance, rapid epidemiology bacteria, there need explore current pattern in our local settings. To achieve t...

Once known some biological characteristics of six Trypanosoma cruzi strains, randomly amplified polymorphic DNA (RAPD) analysis was made. Cluster analysis by UPGMA (unweighted pair group method analysis) was then applied both to biological parameters and RAPD profiles. Inspection of the UPGMA phenograms indicates identical clusters, so supporting that usefulness of biological parameters to char...

Wild olive (O. europaea ssp cuspidata) plants grow in various regions of Iran and are expected to have considerable genetic diversity due to adaptation to the various environmental conditions. We examined the genetic diversity of four populations of wild olive growing in Hormozgan Province located in southern Iran by using 30 RAPDs and 10 ISSR markers. The mean value of polymorphism for RA...

The incidence and transmission patterns of extended-spectrum-beta-lactamase (ESBL)-producing Klebsiella pneumoniae in patients admitted to the intensive care unit (ICU) of a university hospital were investigated over a 3-year period. K. pneumoniae isolates were characterized by antibiotic susceptibility, capsular serotyping, plasmid profiles, and pulsed-field gel electrophoresis (PFGE) of genom...

Objective: This study explored the effects of polysaccharides (RAPD) extracted from traditional anti-stone Chinese medicine Rhizoma alismatis and their carboxymethylated derivatives (RAPs) on crystal phase, morphology, size calcium oxalate (CaOx). It also determined damaging ability regulated crystals human renal tubular epithelial cells (HK-2). Methods: RAPD carboxymethylation with a carboxyl ...

Abstract Background Previous studies have reported conflicting findings between serum anti-citrullinated protein antibodies (ACPA) levels in rheumatoid arthritis (RA) participants with and without periodontitis (Pd). This study aimed to analyse possible correlations ACPA clinical parameters Pd RA participants. Methods Full mouth periodontal examination (probing pocket depth, attachment levels, ...

We have used a "two-way pseudo-testcross" mapping strategy in combination with the random amplified polymorphic DNA (RAPD) assay to construct two moderate density genetic linkage maps for species of Eucalyptus. In the cross between two heterozygous individuals many single-dose RAPD markers will be heterozygous in one parent, null in the other and therefore segregate 1:1 in their F1 progeny foll...

Techniques such as arbitrarily primed polymerase chain reaction (APPCR) (11), random amplified polymorphic DNA (RAPD) (12) and DNA amplification fingerprinting (DAF) (7) are powerful tools for genetic mapping, taxonomic and phylogenetic investigations and for the detection of various types of DNA damage and mutation (2,3). Nevertheless, the arbitrary nature of the DNA polymerization catalyzed i...

DNA fingerprinting has been useful for genotypic classification of American cranberry (Vaccinium macrocarpon Ait.). Polymerase chain reaction (PCR) based methodologies including randomly amplified polymorphic DNA (RAPD) markers are relatively easy to use, and inexpensive as compared to other methods. However, RAPD markers have some limitations including seamless interlaboratory transferability ...

The polymerase chain reaction (PCR) based random amplified polymorphic DNA (RAPD) assay, morphological, physiological, biochemical and antimicrobial susceptibility test methods have been evaluated for use in the taxonomy of isolated thermotolerant Bacillus from Jordanian hot springs, with specific reference to strains Geobacillus stearothermophilus (ATCC 12980), Bacillus circulans (ATCC 4513) a...