Flow cytometry was used to determine the daunomycin con tent of rat bone marrow cells after incubation in vitro. The spontaneous fluorescence of daunomycin was measured upon excitation with laser light at 488 nm. Forward and perpendicular light scatters of the cells were simultaneously measured to allow identification of granulocytic and lymphocytic subpopulations. A linear relationship was fou...

The interaction between histone and nonhistone chromatin proteins has been investigated by histone affinity chromatography, a technique that avoids precipitation of histone l nonhistone complexes. When nonhistone proteins labeled with 32P are loaded directly on histone-Sepharose columns, no binding of radioactivity to the column is observed. However, if labeled nonhistone proteins are mixed wit...

background: investigation on the natural radionuclides 238u, 232th, 40k and natural fallout radionuclide 210po in the prominent plants species of western ghats tropical forest near kotagiri have been carried out as a part of baseline background radiation studies in the forest environment. materials and methods: the prominent plants species of the region evodia roxburghiana and eleaocarpus oblan...

In certain lines of hepatoma tissue-culture cells, the extracellular glutamine concentration regulates the specific activity of glutamine synthetase. By quantifying the radioactivity in immunoprecipitated glutamine synthetase on polyacrylamide gels, we found that the rate of degradation, but not of synthesis, of glutamine synthetase is a sensitive function of extracellular glutamine. The activi...

1. The involvement of intracellular 3':5'-cyclic AMP in the inhibition of platelet aggregation by prostaglandin E(1), isoprenaline and adenosine has been examined by a radiochemical technique. Platelet-rich plasma was incubated with radioactive adenine to incorporate (14)C radioactivity into platelet nucleotides. Pairs of identically treated samples were taken, one for the photometric measureme...