Protein degradation is an essential and highly regulated process. The proteasomal degradation of the tumor suppressors p53 and p73 is regulated by both polyubiquitination and by an ubiquitin-independent process. Here, we show that this ubiquitin-independent process is mediated by the 20S proteasomes and is regulated by NQO1. NQO1 physically interacts with p53 and p73 in an NADH-dependent manner...

introduction: natural organic matters (noms) existing in water resources led to various problems such as formation of disinfectant by-products (dbps). humic matters like humic acid (ha) are component of noms that should be removed from water. advanced oxidation process (aops) is one of the noms removal methods. the aim of this study was to survey the degradation of humic acid by electron beam i...

p53-mediated increase in cyclin-dependent kinase inhibitor p21(WAF1) protein is thought to be the major mediator of cell cycle arrest after DNA damage. Previously p21 protein levels have been reported to increase or to decrease after UV irradiation. We show that p21 protein is degraded after irradiation of a variety of cell types with low but not high doses of UV. Cell cycle arrest occurs despi...

Tumor suppressor p53 is either lost or mutated in several types of cancer. MDM2 interaction with p53 results in ubiquitination and 26S proteasomal degradation of p53. Chronic DNA damage leads to inactivation of MDM2, stabilization of p53, and apoptotic cell death. Here, we present a novel MDM2/ubiquitination-independent mechanism of stabilization and transient activation of p53. The present stu...

The nuclear industry and other high-radiation environments often need remote monitoring equipment with advanced cameras to achieve precise control operations. CMOS image sensors, as a critical component of these cameras, get exposed γ-ray irradiation while operating in such environments, which causes performance degradation that adversely affects camera resolution. This study conducted total io...

Image fiber bundles are employed in fusion experiments and other high radiation environments where they are used to transmit an image from an unprotected objective lens to a radiation shielded camera. Due to their exposure to neutron and gamma radiation the transmission of these expensive image fiber bundles can rapidly degrade, especially at the shorter visible wavelengths, and require costly ...

A degradation study of diazepam (DZP) in aqueous media by gamma radiation, high frequency ultrasound, and UV radiation (artificial-solar), as well with each process intensified oxidizing agents (H2O2 Fenton reagent) was performed. The parameters that influence the such potency frequency, irradiation dose, pH concentration used were studied. Gamma performed a 60Co source irradiator; an 11 W lamp...

It is well known that degradation of optical components is to be expected after prolonged exposure to the conditions experienced in orbit. In particular it is expected that the instrument response to ultra-violet light will change. GOME and SCIAMACHY are prone to degradation. In order to minimise the impact on the scientific data products, several means of monitoring possible degradation were i...

Using solar panels is one of the cleanest ways to generate electricity ever created by mankind. The efficiency rapidly expanding decreases during their lifetime for several reasons, such as photodegradation, hot spots, potentially induced degradation, etc. Dirt and debris accumulation on surface can also significantly contribute performance degradation due diminishing radiation reaching active ...

As a critical ubiquitin ligase, the anaphase-promoting complex/cyclosome (APC/C) governs cell cycle progression, signaling modulation and the pathogenesis of some human diseases. Recent studies implicate APC in maintaining genomic integrity, but the mechanism by which it plays such a role remains largely unknown. We report here that acute UV radiation triggers proteolysis of CDH1, an activator ...