The structure of hyphal walls of Pythium debaryanum was investigated by electron microscopy of shadowed replicas and thin sections, before and after digestion by snail gut enzymes or by 1 n HCl at 100 C for 1 hr, and by X-ray diffraction. We found that the wall had two phases, one composed of microfibrils of unknown composition and a second consisting of an amorphous matrix, part of which stain...

Los Hongos Formadores de Micorrizas Arbusculares (HFMA) ayudan al intercambio y movilidad nutrientes asociarse con plantas, mejorando los cultivos. Por tanto, el objetivo fue evaluar HFMA en dos variedades caña azúcar para panela la localidad Suaita, Santander. Se estableció un diseño bloques azar, tres repeticiones cuatro tratamientos (T1: Acaulospora mellea, T2: Rhizoglomus irregulare, T3: Co...

This study examined fungal diversity and composition in conventional (CM) and desert farming (DE) systems in Oman. Fungal diversity in the rhizosphere of tomato was assessed using 454-pyrosequencing and culture-based techniques. Both techniques produced variable results in terms of fungal diversity, with 25% of the fungal classes shared between the two techniques. In addition, pyrosequencing re...

Studies by Western blot analyses have shown that antibodies in the sera of host species infected by Pythium insidiosum recognized several prominent proteins expressed by this fungus-like pathogen. Although these studies have utilized sera from infected patients and relevant local strains of P. insidiosum, the results are difficult to compare because of the lack of method standardization. In an ...

Pythium species are morphologically polymorphic, physiologically unique and ecologically versatile, which make them significant both theoretically and practically. They are ubiquitous in soil and in water, distributed worldwide, and with very diverse host ranges. They include some of the most important and destructive plant pathogens, causing losses of seeds, pre-emergence and post-emergence da...

Pythium insidiosum, the only species in the genus that infects mammals, is the etiological agent of pythiosis, a granulomatous disease characterized by cutaneous and subcutaneous lesions and vascular diseases. Accurate diagnosis of pythiosis and identification of its causal agent are often inconsistent with current immunological diagnostic methods. A species-specific DNA probe was constructed b...

Pythiosis is an infectious disease caused by Pythium insidiosum, a fungus-like organism. Due to the lack of ergosterol on its cell membrane, antibiotic therapy is ineffective. The conventional treatment is surgery, but lesion recurrence is frequent, requiring several resections or limb amputation. Photodynamic therapy uses photo-activation of drugs and has the potential to be an attractive alte...