Colloidal quantum dots (QDs) afford huge potential in numerous applications owing to their excellent optical and electronic properties. After the synthesis of QDs, separating QDs from unreacted impurities in large scale is one of the biggest issues to achieve scalable and high performance optoelectronic applications. Thus far, however, continuous purification method, which is essential for mass...

Previously, we reported a non-chromatographic protein purification method exploiting the highly specific interaction between the dockerin and cohesin domains from Clostridium thermocellum and the reversible aggregation property of elastin-like polypeptide (ELP) to provide fast and cost-effective protein purification. However, the bound dockerin-intein tag cannot be completely dissociated from t...

Cerium, neodymium and gadolinium contain double beta active isotopes. The most interesting are Nd and Gd (promising for 02 search), Ce (2 candidate with one of the highest Q2). The main problem of compounds containing lanthanide elements is their high radioactive contamination by uranium, radium, actinium and thorium. The new generation 2 experiments require development of methods for a de...

Growing applications of phenylalanine dehydrogenase (PheDH) enzyme in the medical and pharmaceutical industries encourages researchers to seek simple, fast and economical alternative purification methods. With goal of finding a new technique, the extraction and purification of recombinant Bacillus badius PheDH in polyethylene glycol 6000 (PEG-6000) and ammonium sulfate aqueous two-phase systems...

Several prophylactic human papillomavirus (HPV) vaccines have been developed based on virus-like particles (VLPs) made from viral L1 proteins. A substantial number of VLPs is necessary for biochemical characterization and diagnostic test development. To establish the optimum conditions for production and purification of HPV L1 in the yeast expression system we varied the amount and nature of th...

Affinity purification is based on the selective and reversible interaction between two binding partners, of which one is bound to a chromatography matrix and the other may be either a native target protein or a recombinant protein fused with an affinity tag (Cuatrecasas et al. 1968). Recombinant DNA-technology allows straightforward construction of gene fusions to provide fusion proteins with t...

Photochemical reactions with the participation of iron(iii) carboxylates are important for environmental photochemistry and have a great potential of application in water purification (advanced oxidation processes, photo-Fenton and Fenton-like processes). In spite of this, information about excited states and primary intermediates in the photochemistry of Fe(iii) complexes with carboxylic acids...

* Correspondence: xlchen@sicau.edu.cn

A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG™, is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.