A radioirnmunoassay for SP1 (“pregnancy-specific” beta1-glycoprotein) is described in which a sensitivity of 0.1 tg/L was achieved by use of an antiserum with Ka 1.7 X lOll L/mol and delayed addition of a 1251-SP1tracer (specific acty, 25 Ci/g) that was stable for use in the assay for at least six to seven weeks. We also report an additional anti-SP serum, from an early bleeding, that displayed...

ferent breed makeups. It is widely used in commercial beef production because of the benefits it has to offer cow-calf producers. Improvements in efficiency can be dramatic if appropriate breed combinations are used. Crossbreeding does not eliminate the need for outstanding purebred cattle since efficient systems require well characterized, superior purebred cattle. Crossbreeding in beef cattle...

A total of 648 purebred Holstein and 319 backcross Holstein × Jersey dairy cattle were compared for production, reproduction, health, linear type, and growth traits. Animals were born between 2003 and 2009 and were housed in the University of Wisconsin-Madison Integrated Dairy Facility. All animals had Holstein dams; lactating dams were mated to unproven Holstein sires to produce purebred (cont...

Glycoprotein G (gG) of alphaherpesviruses has been described to function as a viral chemokine binding protein (vCKBP). More recently, mutant viruses devoid of gG have been shown to result in increased virulence, but it remained unclear if gG's potential to serve as a vCKBP is responsible for this observation. We here used equine herpesvirus type 1 (EHV-1) as a model to study the pathophysiologi...

Based on a variety of recent findings, the Grueneberg ganglion (GG) in the vestibule of the nasal cavity is considered as an olfactory compartment. However, defined chemical substances that activate GG neurons have not been identified. In this study, the responsiveness of murine GG cells to odorants was examined by monitoring the expression of the activity-dependent gene c-Fos. Testing a number...

During infection of a new host, the first surfaces encountered by herpes simplex viruses are the apical membranes of epithelial cells of mucosal surfaces. These cells are highly polarized, and the protein composition of their apical and basolateral membranes are very different, so that different viral entry pathways have evolved for each surface. To determine whether the viral glycoprotein G (g...

The mammalian olfactory sense employs several olfactory subsystems situated at characteristic locations in the nasal cavity to detect and report on different classes of odors. These olfactory subsystems use different neuronal signal transduction pathways, receptor expression repertoires, and axonal projection targets. The Grueneberg Ganglion (GG) is a newly-appreciated olfactory subsystem with ...

There is evidence for glycine and GABA(A)-receptor-mediated inhibition of hypoglossal motoneurons in vitro. However, comparable studies have not been performed in vivo, and the interactions of such mechanisms with integrative reflex respiratory control have also not been determined. This study tests the hypotheses that glycine at the hypoglossal motor nucleus (HMN) will suppress genioglossus (G...

Many organisms accumulate compatible solutes in response to salt or desiccation stress. Moderate halotolerant cyanobacteria and some heterotrophic bacteria synthesize the compatible solute glucosylglycerol (GG) as their main protective compound. In order to analyse the potential of GG to improve salt tolerance of higher plants, the model plant Arabidopsis thaliana was transformed with the ggpPS...