A case of septic arthritis of the knee caused by Pseudomonas cepacia following an intraarticular corticosteroid injection in a patient with a history of osteoarthritis is presented. This is the second report of this agent causing infection in a diarthrodial joint, which proved difficult to eradicate despite in vitro antibiotic sensitivity.

The genus Burkholderia is currently composed of many species, but only three are notable pathogens for humans or animals: the former cepacia complex (described in Chapter 220) pseudomallei (the agent of melioidosis), and mallei (the agent of equine glanders). All three are aerobic, nonsporulating, straight or slightly curved gram-negative bacilli that were formerly placed in the genus Pseudomonas.

The O9 antigen of Burkholderia (Pseudomonas) cepacia has the following disaccharide repeating-unit: -->4)-alpha-D-Glcp-(1-->3)-alpha-L-Rhap-(1--> The same unit is present in the O antigens of Serratia marcescens strain S1254 and serogroup O4 (predominantly acetylated at O-2 of rhamnose in the latter case).

The aim of the study was to assess microbial quality of water in dental unit waterlines in a dental school at Mangalore. Methodology: Water samples were taken from tap water for control, air/water syringe and the high speed hand piece of 20 dental units. The samples were transferred to the laboratory and streaked on the culture media namely Mac Conkey agar, Sabourauds dextrose media, Buffered c...

The main objective of this study is the enantioselective synthesis carboxyhydroxyphosphonates by lipase-catalyzed reactions. For purpose, racemic dimethyl and dibutyl 1-butyryloxy-1-carboxymethylphosphonates were synthesized hydrolyzed, using a wide spectrum commercially available lipases from different sources (e.g., fungi bacteria). best hydrolysis results 1-butyryloxy-1-carboxymethylphosphon...

Pseudomonas aeruginosa and Burkholderia cepacia are the two major Gramnegative rods that colonize/infect the lungs of patients with cystic fibrosis (CF). These organisms may cause progressive respiratory failure, although occasionally more rapid infections result in the ‘Cepacia’ syndrome. Many antibiotics have been used against Pseudomonas and Burkholderia, but once chronic colonization has be...

The two-component nonheme iron dioxygenase system 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS catalyzes the double hydroxylation of 2-halobenzoates with concomitant release of halogenide and carbon dioxide, yielding catechol. The gene cluster encoding this enzyme, cbdABC, was localized on a 70-kbp conjugative plasmid designated pBAH1. The nucleotide sequences of cbdABC and flan...

A total of 258 respiratory tract specimens from patients with cystic fibrosis were inoculated onto nine different plated media, and the rates of recovery of potential pathogens were compared. Media included sheep blood agar, enriched chocolate agar, MacConkey agar for gram-negative bacilli, chocolate agar containing bacitracin for Haemophilus spp., bromcresol green agar for yeasts, cetrimide ag...

Lipases from Geotrichum candidum 4013 (extracellular lipase and cell-bound lipase) were immobilized by adsorption on chitosan beads. The enzyme preparations were tested in the synthesis of ester prodrugs from racemic 9-(2,3-dihydroxypropyl)adenine in dimethylformamide with different vinyl esters (acetate, butyrate, decanoate, laurate, palmitate). The transesterification activities of these immo...