Expression of genes coding for wound-inducible cathepsin D inhibitor (CDI) and proteinase inhibitor II (PI-II) of petato were induced in leaves upon treatment with methy} jasmonate (MJA) with similar time and concentrat;on dependences. The response of CDI and PI-Il genes to yarious compounds structural]y related to MJA w'ere a}so simi]ar to each other. For the induction of expression of these p...

Molecular detection of pathogenic microorganisms in ticks is based on DNA amplification of the target pathogen; therefore, extraction of DNA from the tick is a major step. In this study, we compared three different tick DNA extraction protocols based on an enzymatic digestion by proteinase K followed by DNA extraction by a commercial kit (method 1), or on mortar crushing, proteinase K digestion...

1. Human uterine cervical stroma was found to contain a Ca(2+)-independent neutral proteinase against casein and N-benzoyl-dl-arginine p-nitroanilide (Bz-dl-Arg-Nan). This enzyme was tightly bound to an insoluble material (20000g pellet) and was solubilized by high concentrations of NaCl or KCl. High concentrations of them in the reaction system, however, inhibited reversibly the activity of th...

This research is aimed to eradicate the biofilm formed by bacteria causing wound infection through using proteinase K enzyme. For this purpose six different concentrations of were used for degradation produced Staphylococcus aureus and Pseudomonas areuginosa. These two species are most common abundant biofilm. Each was kept in contact with pathogenic 1, 2 three hours. After 3 hours incubation p...

BACKGROUND AND OBJECTIVES Human leukocyte elastase, proteinase 3 and cathepsin G are neutrophil granule proteins belonging to the hematopoietic serine protease superfamily. In addition to their established roles in inflammation, they have recently been implicated as regulators of granulopoiesis and mediators of apoptosis. We set out to characterize the individual biosynthetic profiles of these ...