نتایج جستجو برای: protein solubility
تعداد نتایج: 1253247 فیلتر نتایج به سال:
1. The conditions under which the phase rule may be applied to systems containing proteins are formulated. 2. An attempt was made to fractionate chymotrypsinogen, by crystallization in stages with increasing concentration of magnesium sulfate. No significant fractionation of the protein was achieved, but a small amount of impurity which affects the solubility, while having little influence on o...
in this study, the ion association phenomenon in the saturated solution of kcl in the mixed solvent(methanol+water) was studied. the solubility of this compound in the mixed solvent (methanol+water by value percent of methanol = 60%), and in the presence of various molarities of nano3 wasdetermined by the solvent evaporation method at 25.0°c. the results enable us to estimate the valueof thermo...
Functional properties of protein from mantle and fin of the jumbo squid Dosidicus gigas were explained based on microscopic muscle fiber and protein fractions profiles as observed in SDS-PAGE. Fin has higher content of connective tissue and complex fiber arrangement, and we observed higher hardness of fin gels as expected. Myosin heavy chain (MHC) was found in sarcoplasmic, myofibril and solubl...
Producing a well behaved sample at high concentration is one of the main hurdles when starting a new project on an interesting protein. Especially when one attempts to overexpress a eukaryotic protein in bacteria, some difficulties are encountered, such as low expression level, low solubility, or even lack of folded structure. Overexpression in prokaryotic systems is highly desirable for cost-e...
A prerequisite for structural genomics and related projects is to standardize the process of gene overexpression and protein solubility screening to enable automation for higher throughput. We have tested a methodology to rapidly subclone a large number of human genes and screen these for expression and protein solubility in Escherichia coli. The methodology, which can be partly automated, was ...
Coexpression of the enzyme, protein disulfide isomerase (PDI), has been shown to increase soluble and secreted IgG levels from baculovirus-infected insect cells (Hsu, T.-A., Watson, S., Eiden, J. J., and Betenbaugh, M. J. (1996) Protein Expression Purif. 7, 281-288). PDI is known to include catalytic active sites in two separate thioredoxin-like domains, one near the amino terminus and another ...
cottonseed is considered among most important crops. it contains about 26 percent oil that in used for vegetable oil production. after oil extraction, the remaining part of the seed know as cottonseed cake with 45 percent protein is routinely used as a protein supplement in feed rations for livestock and less for food use due to presence of gossypol, a toxic constituent of cottonseed. in t...
Whey protein concentrate (WPC) has many applications in the food industry. Previous research demonstrated that treatment of whey proteins with high hydrostatic pressure (HHP) can enhance solubility and foaming properties of whey proteins. The objective of this study was to use HHP to improve functional properties of fresh WPC, compared with functional properties of reconstituted commercial whey...
Abstract This work evaluated the functional properties of sesame protein fractions in order to determine their potential food applications. Sesame seed were prepared according solubility: water-soluble (albumin), salt-soluble (globulin), alkaline-soluble (glutelin) and ethanol-soluble (prolamin). Globulin was most abundant fraction, consisting 91% protein, followed by glutelin, albumin prolamin...
The objective of this study was to characterize the solubility, structural, thermal, and aggregation properties oat protein under high-intensity ultrasound (HIU) treatment. Suspensions isolate (OPI) at pH 2.0–8.0 were subjected HIU for 5 min 70% amplitude; particle size, surface attributes, UV–Vis absorption, intrinsic fluorescence, polypeptide profiles, calorimetric properties, thermal determi...
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