The large coiled-coil protein NuMA plays an essential role in organizing microtubule minus ends at spindle poles in vertebrate cells. Here, we use both in vivo and in vitro methods to examine NuMA dynamics at mitotic spindle poles. Using fluorescence recovery after photobleaching, we show that an exogenously expressed green-fluorescent-protein/NuMA fusion undergoes continuous exchange between s...

We report that a peripheral Golgi protein with a molecular mass of 210 kD localized at the cis-Golgi network (Rios, R.M., A.M. Tassin, C. Celati, C. Antony, M.C. Boissier, J.C. Homberg, and M. Bornens. 1994. J. Cell Biol. 125:997-1013) is a microtubule-binding protein that associates in situ with a subpopulation of stable microtubules. Interaction of this protein, now called GMAP-210, for Golgi...

Doublecortin (DCX) is a microtubule-associated protein required for neuronal migration to the cerebral cortex. DCAMKL1 consists of an N terminus that is 65% similar to DCX throughout the entire length of DCX, but also contains an additional 360 amino acid C-terminal domain encoding a putative Ca(2+)/calmodulin-dependent protein kinase. The homology to DCX suggested that DCAMKL1 may regulate mic...

An ATPase activity is persistently associated with bovine brain microtubules even after multiple cycles of warm-induced microtubule assembly, centrifugation through a 50% sucrose cushion, cold-induced disassembly, and subsequent centrifugation. Likewise, a sucrose extraction method for preparing microtubule proteins free of mitochondrial contamination does not eliminate ATPase activity in th...

BACKGROUND Mitochondria form a dynamic tubular network within the cell. Proper mitochondria movement and distribution are critical for their localized function in cell metabolism, growth, and survival. In mammalian cells, mechanisms of mitochondria positioning appear dependent on the microtubule cytoskeleton, with kinesin or dynein motors carrying mitochondria as cargos and distributing them th...

The skin provides a barrier function mediated by combination of cornified epithelial cells, cell-cell adhesions and epidermal lipids. is compromised in old age which exemplified features such as improper lipid metabolism flattened dermal-epidermal junction. Laminin 332 basement membrane glycoprotein was shown to be decreased aged skin. We have previously demonstrated that the loss laminin leads...

Abstract Multiple morphological abnormalities of the sperm flagella (MMAF) is one major causes male infertility and characterized by multiple defects. In this study, we found that coiled-coil domain-containing 189 (Ccdc189) gene was predominantly expressed in mouse testes inactivation Ccdc189 caused infertility. Histological studies revealed most from Ccdc189-deficient mice carried coiled, curv...