Deamidation of proteins is probably one of the most common chemical degradation pathways in protein pharmaceuticals. The reaction rate for deamidation of human growth hormone (hGH) is particularly pH-dependent and occurs typically faster at neutral and alkaline pH [1]. Protamine from salmon is an extremely basic peptide due to its high arginine content. It is isolated from sperm cells of salmon...

Protamine sulfate was found to consume large amounts of C selectively during preincubation with sera of individuals in the "acute phase". Marked depletion of C1, C4, and C2 with minimal, if any, depletion of C3-9, was observed. The consumption was time and temperature dependent, occurring most rapidly and extensively at 37 degrees C, 0.10 M relative salt concentration and pH 7.5-8.0; it require...

A protein kinase activity has been found associated with purified Sendai virions. Most of the virion proteins and exogenous protamine served as substrates for this enzyme.

Cardiopulmonary bypass depends on our ability to control the coagulation system. Heparin and protamine have been used for this purpose since the introduction of cardiopulmonary bypass. Although heparin has been used as an anticoagulant since 1935 there are still some unsolved questions as regarding its structure and metabolism. 1•2 Many principles have been proposed for total heparinization, mo...

The ion-step measuring method was used to determine absolute heparin concentrations in PBS and blood plasma with a Ta2O5 ISFET on to which a monolayer of protamine had been immobilized. Heparin is a highly negatively charged polysaccharide, which is used clinically to delay the clotting of blood. Protamine acts as an affinity ligand for heparin. The response of the ISFET system on a step-wise i...

Cationic proteins purified from human polymorphonuclear leukocyte granules exert a cytotoxic effect on mammalian tumor cells. This effect is time and concentration dependent, is inhibited by the anionic agent heparin, and is enhanced by preheating the cationic proteins. Other strongly basic proteins (histone, protamine) also exhibited cytotoxic activity. Myeloperoxidase isolated from human leuk...

One of the main difficulties that the average practitioner encounters regarding the use of msulin in clinical diabetes is the adjustment the proper dosage, on account of insulin having no fixed or standard dosage of its own. The result is that, if proper dosages are not employed, there is either little or no reduction in the blood-sugar level if the dose \s too small, or there is dangerous osci...

Since the publication of a short communication (1) 4 years ago on the binding of sulfate and phosphate to salmine, considerably more information concerning the nature of the molecule is available (2-4). We now present the results of a direct determination of the anion-binding characteristics of salmine, and, in light of the physical and analytical properties, a scheme to account for the fact th...