Various commercial hydrolases were used in an attempt to degrade the endotoxic lipopolysaccharide macromolecule. Some inert components, such as peptides and nucleic acids, could be removed from endotoxin preparations. As a result, endotoxic activity, measured by pyrogenicity, Shwartzman reaction, and mouse lethality, was increased. The remarkable resistance of endotoxin to hydrolases led to the...

It has been shown that several hydrolytic enzymes with different specificities undergo similar reactions with diisopropyl phosphorofluoridate (1) and other related toxic organophosphorus compounds (2). These conclusions were based on kinetic studies, particularly the influence of pH on the rates of reaction, and on inhibition characteristics. Reactions of this type have provided a unique method...

Halophilic enzymes, known as extremozymes produced by halophilic microorganisms, have identical enzymatic features like their non-halophilic counterpart, but they exhibit different properties mainly in structure. Among these, two main points could be mentioned, (i) a high content in acidic amino-acids located predominantly at the protein surface and (ii) requirement for high salt concentration ...

ORIGINAL CONTRIBUTIONS free radicals. If free radicals remain in the phosphate and reduced glutathione were body for longer time, they can damage body obtained from Sisco Co. All other chemicals STUDY OF LEUKOCYTIC HYDROLYTIC ENZYMES IN PATIENTS cells. AMI is frequently associated with and solvents used were of analytical reagent WITH ACUTE STAGE OF CORONARY HEART DISEASE leukocytosis and an el...

Hydrolases constitute a class of enzymes widely distributed in nature from bacteria to higher eukaryotes. The halotolerance of many enzymes derived from halophilic bacteria can be exploited wherever enzymatic transformations are required to function under physical and chemical conditions, such as in the presence of organic solvents and extremes in temperature and salt content. In recent years, ...

The distribution of oxidative and hydrolytic enzyme activities along the nephron of Necturus maculosus Rafinesque was studied histochemically. The proximal tubule possessed all the demonstrable enzyme activities associated with the hexose-monophosphate shunt and glycolysis, but lacked detectable succinic dehydrogenase and cytochrome oxidase activities. Krebs cycle enzymes other than succinic de...

Generalization of the results theoretical and practical research in production beer showed that rise prices for cereals, particular barley, leads to an increase price malt and, accordingly, cost final product – beer. In this regard, modern brewers face acute problem a shortage high-quality raw materials production, as well high competition consumer market. The need inexpensive brewing has grown...