A 10 kb O-antigen gene cluster was sequenced from a Salmonella enterica subsp. enterica Dakar O28 reference strain and from two S. Pomona serogroup O28 isolates. The two S. Pomona O antigen gene clusters showed only moderate identity with the S. Dakar O28 gene cluster, suggesting that the O antigen oligosaccharides may contain one or more sugars conferring the O28 epitope but may otherwise be d...

While much of cancer immunology research has focused on anti-tumor immunity both systemically and within the tumor microenvironment, little is known about the impact of pre-existing malignancy on pathogen-specific immune responses. Here, we sought to characterize the antigen-specific CD8+ T cell response following a bacterial infection in the setting of pre-existing pancreatic adenocarcinoma. M...

Introduction: Infectious diseases are one of the main causes of death worldwide. This has driven scientists to invest in extraction and identification of antimicrobial agents from natural toxins and presentation of novel antibiotics and vaccines. The aim of the current study is to investigate the antibacterial and cytotoxicity effects of the protective antigen domain 4 (PAD4) from Bacillus anth...

INTRODUCTION Hyperacute rejection may be prevented by avoiding the transplantation of kidneys into patients with pre-existing anti-donor Class I human leukocyte antigen antibodies. However, the role of anti-donor-Class II-human leukocyte antigen-DQ antibodies is not established. The question is ever more relevant as more sensitive cross-matching techniques detect many additional antibodies duri...

The role of class II major histocompatibility antigens in classical antigen-presenting cells has been described (Unanue (1984) Annu. Rev. Immunol. 2, 395-428; Watts and McConnell (1987) Rev. Immunol. 5, 461-475). Whether enterocytes, which also express class II antigens, can act as antigen-presenting cells in vivo is not known. One pre-requisite for a role for enterocytes in antigen presentatio...

S-antigen, a highly pathogenic agent for the inducation of experimental autoimmune uveitis ( EAU ), was obtained in a pure state from human retina by conventional salt fractionation, molecular sieve and ion exchange chromatography. The physical and chemical properties of the purified protein including the molecular weight, isoelectric point and amino acid composition were determined. The physic...

Monoclonal antibodies specific for the retinal S-antigen were obtained by hybridization of spleen cells from a BALB/c mouse immunized with bovine S-antigen and NS-1 myeloma cells. Five selected antibodies specifically labeled the photoreceptor cells of the retina by immunofluorescence. Whereas antibody S9E2 only reacted with bovine S-antigen, the other antibodies showed interspecies cross-react...