A sensitive and selective kinetic-spectrophotometric method was developed for the determination of trace amounts of uric acid. The method is based on the inhibitory effect of uric acid on the oxidation of Janus Green by bromate in the presence of sulfuric acid. The reaction was followed spectrophotometrically by measuring the absorbance at 518 nm using the fixed-time method. Under optimum exper...

a sensitive and selective kinetic-spectrophotometric method was developed for the determination of trace amounts of uric acid. the method is based on the inhibitory effect of uric acid on the oxidation of janus green by bromate in the presence of sulfuric acid. the reaction was followed spectrophotometrically by measuring the absorbance at 518 nm using the fixed-time method. under optimum exper...

Poly(acrylic acid) (PAA) chains exhibit conformational change in response to pH, whereas bromate-sulfite-ferrocyanide (BSF) solution shows pH oscillation between 3.2 and 6.6. By use of quartz crystal microbalance with dissipation (QCM-D), we have investigated the pH induced conformational change of PAA brushes under a continuous flow of BSF solution in real time. The changes in frequency and di...

A novel molybdenum-free nitrate reductase was isolated from the obligate chemolithoautotrophic and facultative anaerobic, (halo)alkaliphilic sulphur-oxidizing bacterium Thioalkalivibrio nitratireducens strain ALEN 2. The enzyme was found to contain vanadium and haem c as cofactors. Its native molecular mass was determined as 195 kDa, and the enzyme consists of four identical subunits with appar...