Hallap T. 2005. Assessment of sperm attributes of frozen-thawed AI doses from Swedish and Estonian dairy bull sires. With special reference to pre-selection through swim-up, and the influence of age on potential fertility. Doctoral thesis ISSN 1652-6880, ISBN 91-576-6912-0 The fertility of bull semen used in artificial insemination (AI) is essential for the effective use of this technology in b...

This study was designed to investigate the characteristics of acrosomal changes during capacitation of bovine spermatozoa in relationship to in vitro fertility of individual bulls. Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with or without heparin. The spermatozoa were evaluated in terms of acrosomal changes at 0, 3, 4, 5...

Sperm motility stimulation is a pharmacological treatment used to improve sperm motility and treatment outcome in frozen-thawed epididymal and testicular spermatozoa. Sperm motility is a critical factor in male fertility. Low motility can be caused by a variety factors including abnormal spermatogenesis, oxidative damage, or depletion of intracellular adenosine triphosphate (ATP; molecule that ...

BACKGROUND Leptin and its receptor are present in spermatozoa; however, the role of leptin in sperm function is still controversial. Our present study aimed at demonstrating the effect of cryopreservation on sperm DNA fragmentation (DNAf) and investigating the possible effects of sperm capacitation techniques and leptin in vitro incubation on frozen-thawed sperm DNAf and oxidative stress. MET...

In the present study, the impact of non-functional spermatozoa on the cryopreservation success of functional boar spermatozoa was evaluated. Fifteen sperm-rich ejaculate fractions collected from five fertile boars were frozen with different proportions of induced non-functional sperm (0--native semen sample-, 25, 50 and 75% non-functional spermatozoa). After thawing, the recovery of motile and ...

Background: Cysteine has been used as additive in cryopreservation extenders. It contains of thiol group which plays an antioxidant role to eliminate reactive oxygen species (ROS) during freeze -thaw procedure. Excessive ROS production cause DNA damage and subsequently reduce sperm fertility. On the other hand, protamine protein have high levels of cysteine which is critical in packaging and co...

objective: evaluation of the effects of different doses of antioxidant taurine on oxidative stress and human sperm parameters following cryopreservation. materials and methods: the semen of 20 fertile men were divided to 5 aliquot, one part considered as a fresh after analysis of standard semen parameters (motility, abnormal morphology, viability) and protamine deficiency, dna damage and measu...