Human plasma platelet-activating factor acetylhydrolase (PAF-AH) is a phospholipase A(2) that is primarily associated with low density lipoprotein (LDL). PAF-AH activity has also been found in high density lipoprotein (HDL), although it has recently been indicated that there is no PAF-AH protein in HDL. Plasma paraoxonase 1 (PON1) is an HDL-associated esterase, which also exhibits PAF-AH-like a...

background: growth hormone deficiency (ghd) is associated with an increased cardiovascular mortality.increased oxidative stress has been associated with development of cardiovascular and cerebrovascular diseases. in the present study, we aimed to evaluate oxidant and antioxidant status in patients with ghd by analyzing serum paraoxonase1 (pon1) activity, and malondialdehyde (mda) and thiol leve...

Paraoxonase 1 (PON1) is an HDL bound enzyme which plays a key role in the protection of LDL and HDL from oxidation by hydrolyzing activated phospholipids and lipid peroxide products. Oxidative stress plays a crucial role in the development of atherosclerosis by oxidation of LDL. This study was conducted to determine age-dependent changes in plasma PON1 arylesterase activity and LDL oxidation in...

BACKGROUND Human serum paraoxonase eliminates carcinogenic lipid-soluble radicals. Because expression of the main human paraoxonase gene PON1 varies widely in humans, certain PON1 polymorphisms might be associated with increased risks of cancer. We sought new functional mutations in PON1 and determined whether known or new PON1 mutations were associated with the risk for prostate cancer in a pr...

BACKGROUND Paraoxonase (PON1) enzymatic activity assays are used to characterize sensitivity to organophosphates and oxidative stress. Length of sample storage, temperature and other factors may influence variability of PON1 measurements, especially in longitudinal studies. METHODS Effects of assay temperature, storage duration up to 7 y (-80 degrees C), freeze-thaw cycles, the type of specim...

Background: Paraoxonase-1 (PON1) is one of the HDL-associated proteins which contributes to the antioxidant properties of these lipoproteins. The aim of this pilot study was to evaluate the effect of the nutritional supplement ALAnerv® on serum PON1 activity in post-acute stroke patients undergoing rehabilitation. Methods: We enrolled 28 post-acute stroke patients and randomly divided them into...

BACKGROUND/AIMS Human paraoxonase-1 (PON1) is responsible for the antioxidant effect of high-density lipoprotein (HDL) by inhibiting low-density lipoprotein oxidation. Previous studies discovered dyslipidemia (DL) and decreased PON1 activity in chronic renal failure (CRF). We aimed to determine PON and arylesterase activity, phenotypic distribution of the PON1 enzyme, and lipid profile in low a...

Infants are extremely vulnerable to certain pesticide exposures because they are still developing the ability to produce the enzyme para­ oxonase­1 (PON1), which detoxifies certain organophosphate pesti­ cides such as chlorpyrifos and diazinon. A study published in 2003 indicated that children may reach near­adult levels of PON1 activ­ ity by age 2 years. But a new larger­scale study of partici...

Serum paraoxonase (PON1) is a HDL-associated enzyme exhibiting potentially antiatherogenic properties. Here, we examined the common PON1-192R/Q human polymorphism. Despite numerous studies, the effect of this polymorphism on the antiatherogenic potential of PON1 is yet unresolved. Our structural model suggests that amino acid 192 constitutes part of the HDL-anchoring surface and active site of ...

Infants are extremely vulnerable to certain pesticide exposures because they are still developing the ability to produce the enzyme para­ oxonase­1 (PON1), which detoxifies certain organophosphate pesti­ cides such as chlorpyrifos and diazinon. A study published in 2003 indicated that children may reach near­adult levels of PON1 activ­ ity by age 2 years. But a new larger­scale study of partici...