We have developed a novel electrochemical system for field assisted, fluidic assembly of objects on a microfabricated silicon substrate by means of electrical addressing. The principle of our technique is based on the movement of charged species in solution to oppositely charged electrodes, as seen commonly in electrophoresis. Here, charged species such as beads and cells are moved electrokinet...

The construction industry is growing fast alongside the growth of economy and population. As one major building materials, brick in high demand for purposes. continuous will eventually lead to a shortage raw materials long run, such as sand Ordinary Portland cement. This issue could be resolved by utilizing waste Expanded Polystyrene (EPS) beads Silica Fume (SF), replacement cement production b...

It is difficult to harness the power generated by biological motors to carry out mechanical work in systems outside the cell. Efforts to capture the mechanical energy of nanomotors ex vivo require in vitro reconstitution of motor proteins and, often, protein engineering. This study presents a method for harnessing the power produced by biological motors that uses intact cells. The unicellular, ...

Experiments investigating the local viscoelastic properties of a simple uncross-linked flexible polymer are performed on polyethylene oxide solutions in the semidilute regime using polystyrene beads of varying sizes and surface chemistry as probes. We measure the thermal motions of the beads to obtain the elastic and viscous moduli of our sample. Two different dynamic light scattering technique...

Fig. S1 Layout of the D-DLD with peripheral vacuum pump and electronic connections. (a) The device is fabricated in PDMS and glass using replica molding. (b) Micrographs of the entrance channels and a side-wall. (c) A scale drawing of the separation device. A stream of particles injected into the device is continuously separated about the critical diameter, D c. Particles smaller than the criti...

We describe the design, fabrication, and use of a radio frequency reflectometer integrated with a microfluidic system, applied to the very high-throughput measurement of micron-scale particles, passing in a microfluidic channel through the sensor region. The device operates as a microfabricated Coulter counter [U.S. Patent No. 2656508 (1953)], similar to a design we have described previously, b...

Rapid and precise doping of mesoporous silica beads has been achieved with luminescent quantum dots, leading to 1-2 orders of magnitude improvements over the results previously achieved with polystyrene latex beads. A surprising finding is that multivalent hydrophobic interactions provide a strong driving force for efficient partitioning and immobilization of surfactant-coated quantum dots in t...

An intrinsic uncertainty in particle mass sensing with the suspended microchannel resonator results from variation in a particle's position near the free end of the resonator. To circumvent this error we employ the second flexural bending mode. This mode exhibits additional frequency peaks while particles pass over the antinode, a point where the frequency shift is insensitive to the lateral po...

Solid-state white-light emission was achieved from polystyrene (PS) microbeads incorporated with fluorophores based on perylene bisimide (PBITEG) and oligo(p-phenylenevinylene) (OPV) as acrylic cross-linkers. The PS beads incorporated with only PBITEG gave intense orange-red emission; PS incorporated with OPV exhibited blue-emission, whereas a series of polymers incorporating both cross-linkers...

Given that macrophages can proliferate and that certain microbes survive inside phagocytic cells, the question arises as to the post-mitotic distribution of microbial cargo. Using macrophage-like cells we evaluated the post-mitotic distribution of intracellular Cryptococcus yeasts and polystyrene beads by comparing experimental data to a stochastic model. For beads, the post-mitotic distributio...