objective: pseudomonas aeruginosa is the major cause of septicemia and wound infection in burned patients. immunotraphy is the best practical way for prevention and treatment of these infections. flagella as one of the most important bacterial virulence factors has important role in attachment, motility, chemotaxis and tlr-5-dependent immune response so that it propounded as a vaccine candidate...

the objective of this study was to compare different antigen preparations to produce monoclonalantibodies against bovine leukaemia virus gp51su. the four antigen preparations for immunization ofbalb/c mice were: cl: blv-flk cell lysate, uf: a fraction of cl (between 30 and 100 kda), wvp: wholevirus particles and sp: with ion exchange chromatography, gp51su was semipurified. a total of ninesucce...

background & aim: a feeding trial was conducted to investigate the effect of periodically use of garlic on growth ‎performance, and antibody titer to newcastle and infectious bursal diseases in broiler ‎chickens. experimental: ross broiler chicks randomly allocated into the 10 dietary treatments ‎for 6 weeks. a 3 × 4 factorial experiment was used as three periods of feeding (starter, grower...

The FDA’s Experience with Covid-19 Antibody Tests Early in the pandemic, FDA recognized that ensuring access to antibody tests could advance understanding of and help inform nation...

This study describes the fabrication of an aflatoxin (AFB1) electrochemical immunosensor by immobilization anti-AFB1 antibody-bovine serum albumin (BSA) conjugate on a layer L-cysteine (L-Cys) used to link antibody palladium nanoparticle-boron nitride (PdNP-BN)-modified carbon felt (CF) electrode. Each step was characterized using cyclic voltammetry (CV) and impedance spectroscopy (EIS). These ...

Antibody dynamics on membranes, such as endocytosis and clustering, are vital in determining antibody functions. Yoshiyuki Manabe, Kazuya Kabayama, Koichi Fukase et al. proposed a new approach to modulate its function. In their Communication (e202304779), the was conjugated with galactose-containing N-glycan, internalization suppressed by interaction galectin-3, leading enhancement of complemen...

an experiment was conducted to study the effect of metabolizable energy (me) and protein of japanese quails diets on offspring performance and antibody production against sheep red blood cell (srbc). a total of 432 japanese quails (13 week-old) were divided into nine treatments. each treatment comprised four replicates of twelve birds (9 female and 3 male). nine diets including three levels of ...

infectious bovine rhinotracheitis is a highly contagious disease caused by the bovine herpes virus-1 (bohv-1), resulting in significant losses to livestock around the world. bohv-1 is a major pathogen of cattle, primarily associated with respiratory/genital tract infections and abortion. in the present study, we determined the presence of antibodies in 120 serum samples of cattle with the histo...

introduction: monoclonal antibodies labeled mainly with 99mtc are being widely used as imaging agents in nuclear medicine. recently pr81 was introduced as a new murine anti-muc1 mab against human breast carcinoma. this antibody reacts with the tandem repeat of 20-mer peptide of protein backbone, and is of igg1 class, with an affinity of 2.19×10-8 m-1. due to high specific reactivity, this antib...

results isotype of 1f1 clone was determined to be igg1, which reacted with native protein in the western blot experiment and stained 20% of the membrane of neoplastic cells overexpressing her2 with 3+ grade. however, 3l5 clone showed a low reaction (10%) with native her2 in immunohistochemistry. conclusions the results of both western blotting and immunohistochemistry showed that native her2 ca...