A series of gels were used as model foods to understand relationships among rheological properties and sensory texture. Fracture stress was the best predictor of sensory fracture force and particle breakdown, whereas a measure of the nonlinear region was a better predictor of sensory deformation. INTRODUCTION Food texture is a complex property involving a constant evaluation of the rheological ...

INTRODUCTION SDS-polyacrylamide gel electrophoresis (SDS-PAGE) represents the second-dimension separation of two-dimensional (2D)-PAGE. Large-scale proteome analysis usually requires simultaneous electrophoresis of batches of second-dimension SDS-PAGE gels to maximize the reproducibility of 2D electrophoresis protein profiles. This requirement is most easily met using multiple, vertical second-...

The possibility of immobilization of Acetobacter suboxydans cells by entrapment within polyacrylamide gels or by intercellular cross-linking with glutaral-dehyde was investigated. The oxidation of unsubstituted alditols and aldose diethyl dithioacetals by the resultant modified cells has been achieved.

Proteins from silver-stained gels can be digested enzymatically and the resulting peptides analyzed and sequenced by mass spectrometry. Standard proteins yield the same peptide maps when extracted from Coomassieand silver-stained gels, as judged by electrospray and MALDI mass spectrometry. The low nanogram range can be reached by the protocols described here, and the method is robust. A silver-...

INTRODUCTION The sets of nested DNA fragments generated by DNA sequencing methods are resolved by electrophoresis through thin denaturing polyacrylamide gels. WARNING Large voltages are passed through DNA-sequencing gels at substantial amperages. More than enough current is used in these gels to cause severe burns, ventricular fibrillation, central respiratory arrest, and asphyxia due to paraly...

This protocol describes the detection of small RNAs (~10-200 nucleotides) by blot hybridization. The RNA samples, denatured in formamide, are separated by denaturing polyacrylamide gel electrophoresis. Because high-percentage polyacrylamide gels are required to separate RNAs in this size range, it is necessary to perform electrophoretic transfer to positively charged nylon membranes. After tran...