UNLABELLED Prolyl 4-hydroxylase (EC 1.14.11.2) catalyzes the hydroxylation of -X-Pro-Gly- sequences and plays a central role in the synthesis of all collagens. The [alpha(I)]2beta2 type I enzyme is effectively inhibited by poly(L-proline), whereas the [alpha(II)]2beta2 type II enzyme is not. We report here that the poly(L-proline) and (Pro-Pro-Gly)10 peptide substrate-binding domain of prolyl 4...

Secondary bacterial pneumonias are a frequent complication of influenza and other respiratory viral infections, but the mechanisms underlying viral-induced susceptibility to bacterial infections are poorly understood. In particular, it is unclear whether the host's response against the viral infection, independent of the injury caused by the virus, results in impairment of antibacterial host de...

Polycytidylic acid (poly C) stimulates the activity of deoxycytidylate and deoxyguanylate kinases in cell suspensions of type III pneumococci. Polyadenylic acid (poly A) stimulates the activity of dGMP kinase only. Polyuridylic acid (poly U) or polyinosinic acid (poly I) is inactive in this respect. A selective stimulation of DNA synthesis occurs in comparison to RNA and protein syntheses when ...

The response of free nitrous acid (FNA)-adapted poly-phosphate accumulating organisms (PAOs) to FNA inhibition under aerobic and anoxic conditions was studied. Anoxic P-uptake was 1-6 times more sensitive to the inhibition compared to aerobic P-uptake. The aerobic nitrite reduction rate increased with FNA concentration, accompanied by an equivalent decrease in the oxygen uptake rate, suggesting...

Superhydrophobic particulate films were fabricated via deposition of raspberry-like fluorescent PS/CdTe/silica microspheres on clean glass substrates and surface modification. Particularly, the fluorescent microspheres were prepared by a kind of modified strategy, namely introducing poly (acrylic acid)-functionalized polystyrene microspheres and thiol-stabilized CdTe quantum dots into a hydroly...

Poly(ADP-ribosylation) is a post-translational modification of nuclear proteins typical of most eukaryotic cells. This process participates in DNA replication and repair and is mainly regulated by two enzymes, poly(ADP-ribose) polymerase, which is responsible for the synthesis of polymers of ADP-ribose, and poly(ADP-ribose) glycohydrolase, which performs polymer degradation. The aim of this wor...

A human monoclonal macroglobulin IgM"°", has recently been described which has specificity for poly-a(2-"8)-linked N-acetylneuraminic acid (NeuNAc), 1 the specific capsular polysaccharide of the group B meningococcus and of Escherichia coli Kl (1) . Although studies using this polysaccharide as a vaccine in humans have been unsuccessful (2-4, see reference 5), the serum contained over 20 mg of ...

Deciphering the factors that regulate human neural stem cells will greatly aid in their use as models of development and as therapeutic agents. The extracellular matrix (ECM) is a component of stem cell niches in vivo and regulates multiple functions in diverse cell types, yet little is known about its effects on human neural stem/precursor cells (NSPCs). We therefore plated human NSPCs on four...

We studied the effect of salt addition on a diblock copolymer system with negative Flory–Huggins interaction parameter, χ, indicative attractive interactions between two blocks. The is poly(ethylene oxide)-block-poly(methyl methacrylate) (PEO-PMMA) added lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) salt. asymmetric block copolymers, PEO-PMMA(10–33) and PEO-PMMA(10–64), where numbers refe...