Stroke, the second highest leading cause of death, is caused by an abrupt interruption of blood to the brain. Supply of blood needs to be promptly restored to salvage brain tissues from irreversible neuronal death. Existing assessment of stroke patients is based largely on detailed clinical evaluation that is complemented by neuroimaging methods. However, emerging data point to the potential us...

OBJECTIVES The aim of this study was to prepare control blood for an external quality assessment scheme (EQAS) for international normalized ratio (INR) point-of-care testing (POCT) in the Netherlands and to assess the performance of the participants. METHODS Control blood was prepared from dialyzed pooled patient plasma and washed human erythrocytes. Samples of control blood were mailed to pa...

A new, highly sensitive fluorescence immunoassay for a TIRF (total internal reflection)-based point-of-care testing (POCT) device was developed for the detection of procalcitonin (PCT), a specific and early marker for sepsis and microbial infections. The immunoassay was performed on a bench-top system that fulfilled all the necessary characteristics of a POCT application, including a short meas...

Francisella tularensis is a potential biowarfare/bioterrorism agent and zoonotic pathogen that causes tularemia; thus, surveillance of F. tularensis and first-level emergency response using point-of-care testing (POCT) are essential. The UPT-LF POCT assay was established to quantitatively detect F. tularensis within 15 min, and the sensitivity of the assay was 10(4) CFU · mL(-1) (100 CFU/test)....

There is an increasing incidence of diabetic nephropathy, predominantly in patients with type 2 diabetes (1– 3). Persistent microalbuminuria is a sign of ongoing glomerular injury and a strong predictor of clinical nephropathy, and screening for microalbuminuria, the earliest manifestation of diabetic nephropathy, is recommended for all diabetic patients (4). Point-of-care testing (POCT) offers...

Resumen Objetivos La diabetes mellitus incrementa los riesgos y complicaciones asociadas a la COVID-19. Una de las principales consecuencias pandemia ha sido drástica reducción consultas presenciales. El objetivo este estudio es evaluar el impacto que tenido COVID-19 en gestión determinación HbA 1c sus resultados pacientes ambulatorios adultos pediátricos con diabetes, teniendo cuenta tanto med...

The ELISA is the mainstay for sensitive and quantitative detection of protein analytes. Despite its utility, ELISA is time-consuming, resource-intensive, and infrastructure-dependent, limiting its availability in resource-limited regions. Here, we describe a self-contained immunoassay platform (the "D4 assay") that converts the sandwich immunoassay into a point-of-care test (POCT). The D4 assay...

For point-of-care testing (POCT), a sandwich-typed immunoassay on poly(dimethylsiloxane) (PDMS) microfluidic chips was developed by employing two original technologies: a power-free microchip and laminar flow-assisted dendritic amplification (LFDA). Sequential injection of reagents of immunoassay was carried out by the power-free microchip. In addition, for the amplification of a fluorescent si...

Objective To correlate blood pressure and inflammatory markers with urine albumin analysed with a point-of-care testing (POCT) instrument, nephelometric determination of albumin and creatinine related urine albumin in elderly males. Methods and Results The study population consisted of 103 diabetic and 603 nondiabetic males (age 77 years) in a cross-sectional study in central Sweden. We analyze...

Membrane-based rapid test reagents including immunochromatography are widely used in clinical practice. Recently, high-sensitive based on the method, such as silver amplification method and time resolved fluorescence for influenza testing, has been developed early confirmation of infection can be achieved. Furthermore, genetic automated all steps from extraction till detection, is getting popul...