Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic and tumor antigens has been reported to trigger protective immunity. Objective: To evaluate the use of a DNA immunization strategy for protection against anthrax, a plasmid was constructed. Methods: The partialsequence of protective antigen of Bacillus anthracis, amino acids 175-764, as a potent immunogenic targe...

Background: Crohn's disease and Ulcerative colitis are known as inflammatory bowel disease with high morbidity which are as a result of increasing immune responses to intestinal microbiota in genetically susceptible individuals. The association of adherent invasive Escherichia coli with Crohn's disease in human has been discussed for decades. The principal aim of this study was to assess the re...

CRISPR/Cas9 system is a powerful gene editing tool in vivo and in vitro. Currently, CRISPR/Cas9 delivery cells or tissue with different vehicles are available, and Adeno- associated virus (AAV) in one of them. Due to AAV packaging size limitation, AAV base vectors that carry CRISPR/Cas9 system do not have florescent tag like GFP for simple detection and navigation of cells, containing AAV. The ...

Klebsiella pneumonia is a gram negative bacillus of which it's resistant strains are a common source of nosocomial infections . The goal of this study is determination of drug sensitivity of these resistant strains, and study of causes involved in the resistant especially the presence of plasmid. Ten different strains of klebsiella pneumoniae were isolated from clinical specimens collected from...

In 1988, a new plasmid profile was observed for Vibrio salmonicida isolated from cod (Gadus morhua) and Atlantic salmon (Salmo salar) in fish farms in northern Norway. This new plasmid profile, which consisted of plasmids of 61, 21, 3.4, and 2.8 megadaltons, is 1 of 11 plasmid profiles which have so far been observed for V. salmonicida. Plasmid profiling and plasmid DNA hybridization were used ...

OBJECTIVES To determine the complete nucleotide sequence of the novel Johannesburg β-lactamase-encoding gonococcal plasmid (pEM1) and to determine the strain relatedness of Johannesburg plasmid-containing penicillinase-producing Neisseria gonorrhoeae (PPNG) by molecular typing. METHODS Eleven PPNG isolates containing the Johannesburg β-lactamase-encoding plasmid were previously identified amo...

The base map of domains was drawn using NCBI Conserved Domains searches (http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi). Rep_2, Plasmid replication protein (pfam01719), Viral_Rep, Putative viral replication protein (pfam02407); Gemini_AL1, Geminivirus Rep catalytic domain (pfam00799); Gemini_AL1_M, Geminivirus rep protein central domain (pfam08283); RNA_helicase, RNA helicase (pfam00910)....

Mutants of the killer plasmid of Saccharomyces cerevisiae have been isolated that depend upon chromosomal diploidy for the expression of plasmid functions and for replication or maintenance of the plasmid itself. These mutants are not defective in any chromosomal gene needed for expression or replication of the killer plasmid.--Haploids carrying these mutant plasmids (called d for diploid-depen...