The plasma proteome of healthy dairy cattle and those with footrot was investigated using a shotgun LC-MS/MS approach. In total, 648 proteins were identified in healthy plasma samples, of which 234 were non-redundant proteins and 123 were high-confidence proteins; 712 proteins were identified from footrot plasma samples, of which 272 were non-redundant proteins and 138 were high-confidence prot...

The presence of a low level of M protein in the peripheral blood <3gm/dl which is below the required cut off for the diagnosis of plasma cell myeloma is known as monoclonal gammopathy of undetermined significance (MGUS). Elevated levels of serum M protein and detection of M band on serum protein electrophoresis (SPE) can occur in chronic diseases not related to B cell disorders and not always d...

a simple, rapid and sensitive high-performance liquid chromatographic (hplc) method for the determination of risperidone in human plasma was developed. an hplc system based on a nucleosil c8 column (150?4 mm) and a uv detector (?= 280 nm) were used. a mixture of sodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to ph 6.0 at a flow rate of 1.5 ml min-1 was used as mobile phas...

The objective of current study was to determine the effects of non-thermal atmospheric plasma (NTAP) on physiological features of Black Cumin seeds. Black Cumin seeds were divided into 4 groups: one control group and three experimental groups. The experimental groups were exposed to NTAP for 5, 10 and 20 minutes, respectively. Then the seeds of the experimental and control groups grew for 21 da...

Immunoaffinity depletion with antibodies to the top 7 or top 14 high-abundance plasma proteins is used to enhance detection of lower abundance proteins in both shotgun and targeted proteomic analyses. We evaluated the effects of top 7/top 14 immunodepletion on the shotgun proteomic analysis of human plasma. Our goal was to evaluate the impact of immunodepletion on detection of proteins across d...

A simple, rapid and sensitive high-performance liquid chromatographic (HPLC) method for the determination of risperidone in human plasma was developed. An HPLC system based on a Nucleosil C8 column (150?4 mm) and a UV detector (?= 280 nm) were used. A mixture of sodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to pH 6.0 at a flow rate of 1.5 ml min-1 was used as mobile phas...

PeptideAtlas is a web-accessible database of LC-MS/MS shotgun proteomics results from hundreds of experiments conducted in diverse laboratories, with all data processed via a uniform analysis pipeline. A total of 91 experiments on human plasma and serum are included. Using the PeptideAtlas web interface, users can browse and search the Human Plasma PeptideAtlas for identified peptides and ident...

Experiments have been reported in Paper I (1) in which the proteins of bovine seminal plasma were subjected to preliminary and electrophoretic characterization. In order to characterize this protein system further, ultracentrifugal and immunological methods have been applied. A limited amount of work has been reported on the ultracentrifugation of the seminal plasma proteins. Ross (2) has noted...

Using phytobiotics as fish feed additives has interestingly increased during the recent decade. The aim of the current study was to evaluate the effect of individual and combined levels of thyme and vitamin E in common carp. Juveniles were separately fed four distinct diets including a control diet (commercial diet without any additive), a diet supplemented with 1% ground Shirazi thyme, diet su...