When single cells or tissues are injured, the wound must be repaired quickly in order to prevent cell death, loss of tissue integrity, and invasion by microorganisms. We describe Drosophila as a genetically tractable model to dissect the mechanisms of single-cell wound repair. By analyzing the expression and the effects of perturbations of actin, myosin, microtubules, E-cadherin, and the plasma...

The Golgi apparatus is an essential organelle of the secretory pathway in eukaryotic cells. It processes and transmembrane proteins orchestrates their transport to other endomembrane compartments or plasma membrane. thereby shapes cell surface, controlling polarity, cell-cell communication, immune signaling. cytosolic face hosts regulates signaling cascades, impacting most notably DNA damage re...

The extracellular ionic microenvironment has a close relationship to biological activities such as by cellular respiration, cancer development, and immune response. A system composed of ion-sensitive field-effect transistors (ISFET), cells, and program-controlled fluidics has enabled the acquisition of real-time information about the integrity of the cell membrane via pH measurement. Here we ai...

When the cell membrane is disturbed, phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane. This is one of the earliest signs of apoptosis and can be monitored by the calcium-dependent binding of annexin V. Therefore, annexin V-binding, in conjunction with flow cytometry, was used to evaluate the integrity of the sperm plasma membrane af...

This study was aimed to analyze the metabolic activity and membrane integrity of boar spermatozoa following storage in long-term semen extenders. Boar semen was diluted with Androhep EnduraGuard (AeG), DILU-Cell (DC), SafeCell Plus (SCP) and Vitasem LD (VLD) extenders and stored for 10 days at 17C. Parameters of the analyzed sperm metabolic activity included total motility (TMOT), progressive m...

background: curcumin has shown to affect sperm motility and function in vitro and fertility in vivo. the molecular mechanism(s) by which curcumin affects sperm motility has not been delineated. since modulation of intracellular ph (phi) and plasma membrane polarization is involved in sperm motility, the present study was conducted to investigate the effect of curcumin on these sperm (human and ...