conclusions these results suggest that serum cholesterol, phospholipids and triacylglycerols have no effect on the levels of cholesterol, phospholipids and triacylglycerols of spermatozoa and seminal plasma. our findings suggest that sperm lipid content is regulated locally within the male reproductive tract. patients and methods cholesterol, phospholipids and triacylglycerols in sperm cells, s...

A fast and robust method for lipid profiling utilizing liquid chromatography coupled with mass spectrometry has been demonstrated and validated for the analysis of human plasma. This method allowed quantification and identification of lipids in human plasma using parallel alternating low energy and high energy collision spectral acquisition modes. A total of 275 [corrected] lipids were identifi...

High plasma vitamin C may lower risk of cardiovascular disease as indicated by direct association with plasma high-density-lipoprotein (HDL) cholesterol and HDL2 cholesterol. Plasma lipids and vitamin C were determined in 316 women and 511 men (aged 19-95 y). After adjustment for age, sex, obesity, and smoking, plasma vitamin C was directly associated with HDL- (P = 0.01) and HDL2 cholesterol (...

In a previous paper (1), the synthesis and entry into the plasma of the protein moiety of the different lipoprotein classes has been described. The present studies deal with the metabolism of the free and esterified cholesterol of the lipoproteins. Relatively little is known of the metabolism of the plasma lipids as they exist in the various lipoprotein fractions. Study of certain lipids is dif...

Our view of the lateral organization of lipids and proteins in the plasma membrane has evolved substantially in the last few decades. It is widely accepted that many, if not all, plasma membrane proteins and lipids are organized in specific domains. These domains vary widely in size, composition, and stability, and they represent platforms governing diverse cell functions. The presynaptic plasm...

Rough endoplasmic reticulum, Golgi apparatus, and plasma membrane rich cell fractions were isolated from livers of rats under similar conditions of age and diet for analysis of phospholipid classes and fatty acid composition. Phosphatidylcholine was the major phospholipid of all membrane types. Golgi apparatus was intermediate between endoplasmic reticulum and plasma membrane with respect to le...

This study was designed to investigate the effects of long-term feeding of chitosan on plasma glucose and lipids in rats fed a high-fructose (HF) diet (63.1%). Male Sprague-Dawley rats aged seven weeks were used as experimental animals. Rats were divided into three groups: (1) normal group (normal); (2) HF group; (3) chitosan + HF group (HF + C). The rats were fed the experimental diets and dri...

OBJECTIVE The objective of this study was to evaluate the effect of polymorphism S447X on plasma lipids of patients with premature coronary artery disease (CAD). METHODS Plasma lipids and genotypes were determined in 2 groups: 313 patients with premature CAD (<55 years of age) and 150 controls without CAD. RESULTS Frequency of the S447X polymorphism was 18% in patients with CAD and 23% in t...

A method has been developed for combining lipids in vitro with delipidized high density lipoprotein (apo-HDL) by mixing a concentrated petroleum ether solution of lipids with an aqueous solution of apo-HDL. When a mixture of plasma lipids was used, combination of phospholipids and cholesterol was observed in amounts comparable with those present in native HDL; triglycerides and cholesterol este...