background and aims: a selective and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been established for determination of methotrexate in human plasma. methods: methotrexate was extracted from plasma with acetonitrile. the mobile phase consisted of acetonitrile-water-formic acid 74: 25: 1(v/v). twenty µl of sample was chromatographically an...

As a carcinogenic pollutant, hexavalent chromium (Cr(VI)) in the ambient air has serious influences on public health. Due to its instability and difficulty chemical analysis, few studies have been conducted regional concentration level of environmental Cr(VI), especially China. The pollution levels Cr(VI) PM2.5 were determined two sampling sites Beijing Qingdao from September December 2020. con...

Mass spectrometry (MS) has greatly enhanced research in the field of microbial proteomics. Microbial characterization, including detection, differentiation, and identification, are the most important tasks of microbial proteomic laboratories worldwide.2-DE is a well-established technique for separation and quantitation of a large set of proteins and has much to contribute to the experimental an...

BACKGROUND Quantitative proteomic analysis with mass spectrometry holds great promise for simultaneously quantifying proteins in various biosamples, such as human plasma. Thus far, studies addressing the reproducible measurement of endogenous protein concentrations in human plasma have focussed on targeted analyses employing isotopically labelled standards. Non-targeted proteomics, on the other...

Preeclampsia is a serious complication of pregnancy, which affects 2-8% of all pregnancies and is one of the leading causes of maternal and perinatal mortality and morbidity worldwide. To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used high-resolution LC-MS/MS technologies to construct a comparative N-glycoproteomic and phosph...