BACKGROUND Acetyl-xylan esterase (AXE, EC 3.1.1.72) hydrolyses acetate group from the linear chain of xylopyranose residues bound by β-1,4-linkage. The enzyme finds commercial applications in bio-bleaching of wood pulp, treating animal feed to increase digestibility, processing food to increase clarification and converting lignocellulosics to feedstock and fuel. In the present study, we report ...

The mutant of Pediococcus pentosaceus (SPAm) produced earlier by UV-mutagenesis exhibiting higher dextransucrase activity as compared to wild-type was used. The generated mutant SPAm gave 12.2 mg/ml, a 20% higher dextran than wild-type. Response surface methodology was carried out for further enhancement of dextran production. To enhance dextran production by the mutant SPAm, Plackett-Burman De...

BACKGROUND Although lovastatin production has been reported for different microorganism species, there is limited information about lovastatin production by basidiomycetes. AIMS The optimization of culture parameters that enhances lovastatin production by Omphalotus olearius OBCC 2002 was investigated, using statistically based experimental designs under solid state fermentation. METHODS Th...

The bacterial strain producing thermostable, alklophilic alpha-amylase was identified as Bacillus amyloliquefaciens KCP2 using 16S rDNA gene sequencing data (NCBI Accession No: KF112071). Medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-state fermentation using wheat bran as the substrate. The medium components infl...

Due to cost, time, and flexibility constraints, simulators are often used to explore the design space when developing new processor architectures, as well as when evaluating the performance of new processor enhancements. However, despite this dependence on simulators, statistically rigorous simulation methodologies are not typically used in computer architecture research. A formal methodology c...

In this study, an analytical methodology based on a combination of dispersive liquid–liquid microextraction with laser-induced breakdown spectrometry was evaluated for simultaneous pre-concentration, speciation and detection of Cr. The microextraction procedure was based on the injection of appropriated quantities of 1-undecanol and ethanol into a sample solution containing the complexes formed...

Response surface methodology was engaged for the optimization of diverse nutritional and physical parameters for laccase production by Streptomyces chartreusis strain NBRC 12753 in the submerged fermentation process. Screening of production parameters was executed using Plackett–Burman design and the variables with statistically momentous effects on laccase production were recognized. Variables...

The biofloculant production potential of a consortium of Streptomyces and Brachybacterium species were evaluated. Optimum bioflocculant yields (g/L) and flocculation activities (%) were observed for the following preferred nutritional sources: glucose (56%; 2.78 ± 0.15 g/L), (NH4)2NO3 (53%; 2.81 ± 0.37 g/L) and CaSO4 · H2O (47%; 2.19 ± 0.13 g/L). A Plackett-Burman design revealed the critical f...

Mevastatin, also known as compactin or ML-236B is a member of the class of statins belonging to the polyketide group. Polyketides are rich sources of pharmaceuticals, including antibiotics, anticancer drugs, and cholesterol-lowering drugs, immunosuppressants and other therapeutics [1]. This study was performed for the production of mevastatin using sesame oil cake as substrate with Pencillium c...